Mayerhofer Hubert, Mueller-Dieckmann Christoph, Mueller-Dieckmann Jochen
EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):117-20. doi: 10.1107/S1744309110047640. Epub 2010 Dec 23.
Ethylene, a gaseous plant hormone, is perceived by a group of membrane-bound receptors. Constitutive triple response 1 (CTR1) from Arabidopsis thaliana directly interacts with ethylene receptors and thus links signal reception to the intracellular signalling pathway. The C-terminal protein kinase domain of CTR1 has been crystallized in its wild-type form and as a kinase-dead mutant. The wild-type crystals diffracted X-ray radiation to 3 Å resolution and the crystals of the kinase-dead mutant diffacted to 2.5 Å resolution. The crystals belonged to space groups P4(1)2(1)2 and P4(2)2(1)2, respectively, with two molecules per asymmetric unit in both cases.
乙烯是一种气态植物激素，由一组膜结合受体感知。拟南芥的组成型三重反应1（CTR1）直接与乙烯受体相互作用，从而将信号接收与细胞内信号通路联系起来。CTR1的C末端蛋白激酶结构域已以其野生型形式和激酶失活突变体形式结晶。野生型晶体将X射线辐射衍射到3埃分辨率，激酶失活突变体的晶体衍射到2.5埃分辨率。这些晶体分别属于空间群P4(1)2(1)2和P4(2)2(1)2，两种情况下每个不对称单元均有两个分子。
