Graduation Course in Biomedicine, Santa Casa Hospital, Belo Horizonte, Minas Gerais, Brazil.
Retina. 2011 Jul-Aug;31(7):1392-9. doi: 10.1097/IAE.0b013e318205b249.
To investigate the effect of calcium channel blockers, spider toxins, on cell viability and the glutamate content of ischemic retinal slices.
Rat retinal slices were subjected to ischemia via exposure to oxygen-deprived low-glucose medium for 45 minutes. Slices were either treated or not treated with the toxins PhTx3, Tx3-3, and Tx3-4. After oxygen-deprived low-glucose insult, glutamate content and cell viability were assessed in the slices by confocal and optical microscopy.
In the retinal ischemic slices that were treated with PhTx3, Tx3-3, and Tx3-4, confocal imaging showed a decrease in cell death of 79.5 ± 3.1%, 75.5 ± 5.8%, and 61 ± 3.8%, respectively. Neuroprotective effects were also observed 15, 30, 60, and 90 minutes after the onset of the retinal ischemic injury. As a result of the ischemia, glutamate increased from 6.2 ± 1.0 nMol/mg protein to 13.2 ± 1.0 nMol/mg protein and was inhibited by PhTx3, Tx3-3, and Tx3-4 to 8.6 ± 0.7, 8.8 ± 0.9, and 7.4 ± 0.8 nMol/mg protein, respectively. Histologic analysis of the live cells in the outer, inner, and ganglion cell layers of the ischemic slices showed a considerable reduction in cell death by the toxin treatment.
Spider toxins reduced glutamate content and cell death of retinal ischemic slices.
研究钙通道阻滞剂、蜘蛛毒素对缺血性视网膜切片细胞活力和谷氨酸含量的影响。
通过将氧剥夺低糖培养基孵育 45 分钟使大鼠视网膜切片发生缺血。用 PhTx3、Tx3-3 和 Tx3-4 毒素处理或不处理切片。在氧剥夺低糖损伤后，通过共聚焦和光学显微镜评估切片中的谷氨酸含量和细胞活力。
在用 PhTx3、Tx3-3 和 Tx3-4 处理的视网膜缺血切片中，共聚焦成像显示细胞死亡分别减少了 79.5±3.1%、75.5±5.8%和 61±3.8%。在视网膜缺血损伤发生后 15、30、60 和 90 分钟，也观察到了神经保护作用。由于缺血，谷氨酸从 6.2±1.0 nMol/mg 蛋白增加到 13.2±1.0 nMol/mg 蛋白，PhTx3、Tx3-3 和 Tx3-4 将其抑制到 8.6±0.7、8.8±0.9 和 7.4±0.8 nMol/mg 蛋白。对缺血切片中外、内和节细胞层中存活细胞的组织学分析表明，毒素处理显著减少了细胞死亡。
蜘蛛毒素降低了缺血性视网膜切片的谷氨酸含量和细胞死亡。
