Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Hobart, Australia.
J Sep Sci. 2011 Apr;34(8):877-87. doi: 10.1002/jssc.201000904. Epub 2011 Mar 10.
This HPLC tutorial focuses on the preparation and use of kinetic plots to characterise the performance in isocratic and gradient LC. This graphical approach allows the selection of columns (i.e. optimum particle size and column length) and LC conditions (operating pressure and temperature) to generate a specific number of plates or peak capacity in the shortest possible analysis time. Instrument aspects including the influence of extra-column effects (maximum allowable system volume) and thermal operating conditions (oven type) on performance are discussed. In addition, the performance characteristics of porous-shell particle-packed columns and monolithic stationary phases are presented and the potential of future column designs is discussed.
本 HPLC 教程侧重于制备和使用动力学图来描述等度和梯度 LC 中的性能。这种图形方法允许选择柱（即最佳粒径和柱长）和 LC 条件（操作压力和温度），以在最短的分析时间内生成特定数量的塔板或峰容量。本文讨论了仪器方面的问题，包括柱外效应（最大允许系统体积）和热操作条件（炉型）对性能的影响。此外，还介绍了多孔壳颗粒填充柱和整体固定相的性能特点，并讨论了未来柱设计的潜力。
