State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Pests, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
J Appl Microbiol. 2011 Jun;110(6):1390-401. doi: 10.1111/j.1365-2672.2011.05001.x. Epub 2011 Apr 27.
To (i) identify the bacterial communities in the gut of oriental fruit fly (Bactrocera dorsalis) adult and (ii) determine whether the different surroundings and diets influence the bacteria composition.
Polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) fingerprinting was used to investigate bacterial diversity in the oriental fruit fly adult gut. The 16S rDNA cloned libraries from the intestinal tract of laboratory-reared (LR), laboratory sterile sugar-reared (LSSR) and field-collected (FC) populations of oriental fruit fly were compared. Phylogenetic analysis of 16S rDNA revealed that Gammaproteobacteria were dominant in the all samples (73·0-98·3%). Actinobacteria and Firmicutes were judged to be major components of a given library as they constituted 10% or more of the total clones of such library. The Flavobacteria, Deltaproteobacteria, Bacteroidetes and Alphaproteobacteria were observed in small proportions in various libraries. Further phylogenetic analyses indicated common bacterial phylotypes for all three libraries, e.g. those related to Klebsiella, Citrobacter, Enterobacter, Pectobacterium and Serratia. LIBSHUFF analysis showed that the bacterial communities of B. dorsalis from the three populations were significantly different from each other (P < 0·0085).
(i) The intestinal tract of B. dorsalis adult contains a diverse bacterial community, some of which are stable. (ii) Different environmental conditions and food supply could influence the diversity of the harboured bacterial communities and increase community variations.
Comparison of the microbial compositions and common bacterial species found in this paper may be very important for the biocontrol of B. dorsalis.
（i）鉴定东方果实蝇（Bactrocera dorsalis）成虫肠道中的细菌群落，（ii）确定不同的环境和饮食是否会影响细菌组成。
采用聚合酶链反应-变性梯度凝胶电泳（DGGE）指纹图谱技术研究东方果实蝇成虫肠道中的细菌多样性。比较了实验室饲养（LR）、实验室无菌糖饲养（LSSR）和野外采集（FC）三种东方果实蝇肠道的 16S rDNA 克隆文库。16S rDNA 系统发育分析显示，γ-变形菌门在所有样品中占主导地位（73.0-98.3%）。放线菌门和厚壁菌门被判断为特定文库的主要组成部分，因为它们构成了该文库总克隆数的 10%或更多。在不同文库中，黄杆菌门、δ-变形菌门、拟杆菌门和α-变形菌门的比例较小。进一步的系统发育分析表明，所有三个文库都存在共同的细菌类群，例如与克雷伯氏菌、柠檬酸杆菌、肠杆菌、果胶杆菌和沙雷氏菌相关的类群。LIBSHUFF 分析表明，来自三个种群的 B. dorsalis 细菌群落彼此之间存在显著差异（P < 0.0085）。
（i）东方果实蝇成虫肠道中含有丰富多样的细菌群落，其中一些是稳定的。（ii）不同的环境条件和食物供应可能会影响栖息细菌群落的多样性，并增加群落的变化。
本文比较了微生物组成和常见细菌种类，这对东方果实蝇的生物防治可能非常重要。
