Shi Zhanghong, Wang Lili, Zhang Hongyu
State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Pests, Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2012;13(5):6266-6278. doi: 10.3390/ijms13056266. Epub 2012 May 21.
Our goal was to identify the bacteria inhabiting the reproductive system of the female oriental fruit fly, Bactrocera dorsalis (Hendel), and evaluate the chemotaxis of B. dorsalis to the metabolites produced by the bacteria. Based on 16S rRNA-based polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), 18 operational taxonomic units (OTUs) were assigned to the five bacterial classes Betaproteobacteria, Alphaproteobacteria, Gammaproteobacteria, Bacilli and Actinobacteria. Nine OTUs were assigned to Gammaproteobacteria, which was the most highly represented class. Enterobacteriaceae constituted the dominant family, and within this family, three genera and five species were identified, including Enterobacter sakazakii, Klebsiella oxytoca, Klebsiella pneumoniae, Raoultella terrigena and Enterobacter amnigenus. In this set, the first two species were the dominant components, and the latter three species were the minor ones. Finally, we found that the metabolites produced by R. terrigena, K. oxytoca and K. pneumoniae were attractive to the B. dorsalis adults, and in field studies, B. dorsalis adults were most attracted to K. oxytoca. Collectively, our results suggest that the female reproductive system plays an important role in the transfer of enterobacteria from the gut to fruit. Our data may prompt the development of a female-targeted population control strategy for this fly.
我们的目标是鉴定栖息于雌性东方果实蝇（橘小实蝇，Bactrocera dorsalis (Hendel)）生殖系统中的细菌，并评估橘小实蝇对这些细菌产生的代谢产物的趋化性。基于16S rRNA聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE），18个操作分类单元（OTU）被归为β-变形菌纲、α-变形菌纲、γ-变形菌纲、芽孢杆菌纲和放线菌纲这五个细菌类别。九个OTU被归为γ-变形菌纲，该类别占比最高。肠杆菌科是优势科，在这个科中，鉴定出了三个属和五个种，包括阪崎肠杆菌、产酸克雷伯菌、肺炎克雷伯菌、土生拉乌尔菌和嗜氨肠杆菌。在这一组中，前两个种是主要成分，后三个种是次要成分。最后，我们发现土生拉乌尔菌、产酸克雷伯菌和肺炎克雷伯菌产生的代谢产物对橘小实蝇成虫具有吸引力，并且在田间研究中，橘小实蝇成虫对产酸克雷伯菌最为吸引。总体而言，我们的结果表明，雌性生殖系统在肠道内的肠杆菌向果实的传播中起着重要作用。我们的数据可能会促使针对这种果蝇制定以雌性为目标的种群控制策略。
