Liu Shi-Huo, Chen Yong, Li Wei, Tang Guang-Hui, Yang Yang, Jiang Hong-Bo, Dou Wei, Wang Jin-Jun
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, China.
J Econ Entomol. 2018 Dec 14;111(6):2861-2868. doi: 10.1093/jee/toy231.
The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most destructive agricultural pests and hosts diverse intestinal bacterial communities. We used 16S rRNA gene sequencing to investigate the microbial communities associated with the intestines of females and males from two B. dorsalis populations collected from Hainan and Guizhou Provinces of China. A total of 260,615 high-quality 16S rRNA gene reads with an average length of 253 bp were obtained. Highly diverse bacterial communities were observed across individuals, with communities containing between 691 and 1,262 bacterial operational taxonomic units. In addition, 37 bacterial phyla, 65 classes, 130 orders, 198 families, 201 genera, and 70 annotated species were identified, wherein the Proteobacteria were the most dominant phylum among all of the libraries, followed by the Firmicutes and Bacteroidetes. Bacterial community composition varied by host sex and geographic location of the populations. Female gut communities comprised fewer bacterial families than males, and females had lower relative abundances of 33 of the 35 most abundant families. In addition, female gut communities harbored greater abundances of Enterobacteriaceae than males. The Hainan population gut communities contained fewer bacterial families than those of the Guizhou populations. However, 5 of the 35 most abundant families were more abundant in communities from the Hainan population than those of the Guizhou population. The results of this study help us better understand the importance of symbiotic bacteria in B. dorsalis and provide a foundation for assessing the use of gut microorganisms as bio-control agents for these serious pests.
桔小实蝇（Bactrocera dorsalis (Hendel)）是最具破坏性的农业害虫之一，其肠道细菌群落多样。我们利用16S rRNA基因测序技术，对从中国海南省和贵州省采集的两个桔小实蝇种群的雌雄个体肠道相关微生物群落进行了研究。共获得260,615条高质量的16S rRNA基因序列读数，平均长度为253 bp。在个体间观察到高度多样的细菌群落，群落中包含691至1,262个细菌操作分类单元。此外，还鉴定出37个细菌门、65个纲、130个目、198个科、201个属和70个注释物种，其中变形菌门是所有文库中最主要的门类，其次是厚壁菌门和拟杆菌门。细菌群落组成因宿主性别和种群地理位置而异。雌性肠道群落中的细菌科数量少于雄性，在35个最丰富的科中，雌性有33个科的相对丰度较低。此外，雌性肠道群落中肠杆菌科的丰度高于雄性。海南种群的肠道群落中的细菌科数量少于贵州种群。然而，在35个最丰富的科中，有5个科在海南种群群落中的丰度高于贵州种群。本研究结果有助于我们更好地理解共生细菌在桔小实蝇中的重要性，并为评估利用肠道微生物作为这些严重害虫的生物防治剂提供基础。
