Ikewaki N, Tamauchi H, Yamaura N, Takahashi H, Inoko H
Department of Microbiology, School of Nursing, Kitasato University, Kanagawa.
Jpn J Cancer Res. 1990 Feb;81(2):153-60. doi: 10.1111/j.1349-7006.1990.tb02542.x.
A cell line secreting a human monoclonal antibody was established by Epstein-Barr virus transforming B cells derived from an enlarged cervical lymph node excised from a patient bearing a carotid body tumor. The reactivity of the monoclonal antibody, designated as mNISP, was tested on various cells and cell lines. An antigen defined by the mNISP was expressed on some Burkitt's lymphoma cell lines and on a non-T non-B acute lymphoblastic leukemia cell line. Furthermore, this antigen was expressed on leukemic cells from 2 of 8 patients with chronic myelocytic leukemia, 2 of 10 patients with acute myeloblastic leukemia, one of 13 patients with acute lymphoblastic leukemia, and two patients with adult T cell leukemia, but it was not expressed on normal T, B and adherent (macrophage) cells. In addition, mNISP reacted with T cells obtained from human T-cell leukemia virus type I carriers. We found that the antigen defined by mNISP was distinct from any previously reported antigen in terms of its pattern of cellular expression and molecular weight, suggesting that mNISP recognizes a new antigen expressed on some lymphoid cells.
通过爱泼斯坦 - 巴尔病毒转化从一名患有颈动脉体瘤患者切除的肿大颈部淋巴结中获取的B细胞，建立了一种分泌人单克隆抗体的细胞系。将这种命名为mNISP的单克隆抗体在各种细胞和细胞系上进行反应性测试。mNISP所定义的一种抗原在一些伯基特淋巴瘤细胞系和一种非T非B急性淋巴细胞白血病细胞系上表达。此外，该抗原在8例慢性粒细胞白血病患者中的2例、10例急性髓细胞白血病患者中的2例、13例急性淋巴细胞白血病患者中的1例以及2例成人T细胞白血病患者的白血病细胞上表达，但在正常T细胞、B细胞和贴壁（巨噬细胞）细胞上不表达。另外，mNISP与从I型人T细胞白血病病毒携带者获得的T细胞发生反应。我们发现，mNISP所定义的抗原在细胞表达模式和分子量方面与之前报道的任何抗原都不同，这表明mNISP识别一种在某些淋巴细胞上表达的新抗原。
