Department of Clinical Microbiology and Infectious Diseases, National Hospital Abuja, Nigeria.
Diagn Microbiol Infect Dis. 2011 Apr;69(4):405-9. doi: 10.1016/j.diagmicrobio.2010.10.011.
HIV/AIDS disease is endemic in Nigeria and associated with stigmatization. Availability of a reliable rapid testing kit and procedure will increase uptake of services. The study aimed to determine the correlation between detection of HIV antibodies in blood to that in oral fluid and to determine the sensitivity and specificity of the Dual Path Platform (DPP) testing kit using oral fluid samples. HIV antibodies detected in oral mucosa transudate and whole capillary blood from HIV-positive, high-risk and low-risk participants were compared with results obtained with whole venous blood from the same participants tested with Determine and Western blot (for discordant cases). Oral fluid test has sensitivity and specificity of 100% relative to Determine rapid assay, while whole capillary blood test has sensitivity of 100% and specificity of 99.5%. DPP oral fluid test is a reliable point-of-care test and may be deployed in large-scale screening exercises.
艾滋病在尼日利亚流行，并伴有污名化现象。可靠的快速检测试剂盒和程序的可用性将提高服务的利用率。本研究旨在确定血液中 HIV 抗体与口腔液中的 HIV 抗体的相关性，并确定使用口腔液样本的双通道平台（DPP）检测试剂盒的敏感性和特异性。比较了从 HIV 阳性、高风险和低风险参与者的口腔黏膜渗出物和全毛细血管血中检测到的 HIV 抗体与用 Determine 和 Western blot（用于不一致的病例）从相同参与者的静脉全血中获得的结果。口腔液检测相对于 Determine 快速检测的敏感性和特异性为 100%，而全毛细血管血检测的敏感性为 100%，特异性为 99.5%。DPP 口腔液检测是一种可靠的即时检测方法，可用于大规模筛查。
