Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.
Theriogenology. 2011 Jul 1;76(1):115-25. doi: 10.1016/j.theriogenology.2011.01.024. Epub 2011 Mar 11.
Since antioxidants can overcome the negative effects of reactive oxygen species (ROS) during sperm cryopreservation, post-thaw sperm quality in flat-headed cats (Prionailurus planiceps), an endangered species, might benefit from the addition of antioxidants to semen extender. The objectives of this study were to: 1) investigate semen traits; and 2) evaluate effects of the vitamin E analogue Trolox (vitamin E) and glutathione peroxidase (GPx) on the quality of frozen sperm from captive flat-headed cats in Thailand. Eight ejaculates were collected by electroejaculation from four flat-headed cats. Each semen sample was divided into three aliquots and re-suspended in a semen extender as follows: 1) without antioxidant supplementation (control); 2) supplemented with 5 mM vitamin E; or 3) supplemented with 10 U/mL GPx. All samples were cryopreserved and thawed. Subjective sperm motility, progressive motility, and the integrity of the sperm membrane, acrosome and DNA were evaluated at semen collection, after 1 h cold storage, and at 0 and 6 h after thawing. Mitochondrial membrane potential, early apoptotic cells, and embryo development by heterologous in vitro fertilization were evaluated after thawing. Captive flat-headed cats were affected by teratozoospermia. After 1 h cold storage, sperm membrane integrity in samples supplemented with GPx was higher than the control group (54.5 ± 13.7 vs 51.3 ± 13.9; P < 0.05; mean ± SD). Sperm frozen in extender with GPx had higher motility at 6 h and greater mitochondrial membrane potential at 0 and 6 h post-thaw incubation than the other groups (P < 0.05). In conclusion, GPx improved the quality of frozen-thawed sperm in flat-headed cats.
由于抗氧化剂可以克服精子冷冻保存过程中活性氧（ROS）的负面影响，因此添加抗氧化剂到精液稀释液中可能会提高扁头猫（Prionailurus planiceps），一种濒危物种，解冻后精子的质量。本研究的目的是：1）研究精液特性；2）评估生育酚类似物 Trolox（维生素 E）和谷胱甘肽过氧化物酶（GPx）对来自泰国圈养扁头猫的冷冻精子质量的影响。通过电刺激从四只扁头猫中采集了 8 个精液样本。每个精液样本分为三部分，分别悬浮在以下三种精液稀释液中：1）不添加抗氧化剂（对照组）；2）添加 5 mM 维生素 E；或 3）添加 10 U/mL GPx。所有样本均进行冷冻保存和解冻。在精液采集时、1 小时冷藏后以及解冻后 0 小时和 6 小时评估精子的主观活力、前向运动活力和精子膜、顶体和 DNA 的完整性。解冻后评估线粒体膜电位、早期凋亡细胞和异种体外受精胚胎发育情况。圈养的扁头猫受到畸形精子症的影响。在 1 小时冷藏后，添加 GPx 的样本中的精子膜完整性高于对照组（54.5 ± 13.7 对 51.3 ± 13.9；P < 0.05；平均值 ± 标准差）。与其他组相比，在含有 GPx 的稀释液中冷冻的精子在解冻后 6 小时具有更高的活力，并且在解冻后 0 小时和 6 小时具有更高的线粒体膜电位（P < 0.05）。总之，GPx 提高了扁头猫冷冻解冻精子的质量。
