Department of Neurology, Southern Medical University, Guangzhou, 510515, China.
Brain Res. 2011 May 10;1389:27-34. doi: 10.1016/j.brainres.2011.03.007. Epub 2011 Mar 9.
This study was carried out to investigate whether the congenital HCMV infection affect the induction and maintenance of LTP /DP. Rat models of Sprague-Dawley rats congenitally infected by HCMV were made. Field excitatory postsynaptic potentials (EPSPs) were recorded in the hippocampal slices of offspring rats (50-65days) to study alterations of LTP /DP in area dentate gyrus (DG) of the hippocampus after congenital infection. The Ca(2+) and mRNA level of calmodulin (CaM) in the hippocampus neurons of the experiment group (congenital infected by HCMV) and the control group were measured;The input/output (I/O) curves of the EPSP slope PS amplitude in area DG in experiment group were significantly depressed when compared to control group (P<0.05). LTP of the EPSP slope and PS amplitude in area DG of the hippocampus was 137±4% (EPSP) and 225±11% (PS) in control rats and 115±9% (EPSP) and 163±7% (PS) in experiment rats (EPSP: F=25.29,P<0.05;PS: F=74.33 P<0.05, two-way ANOVA with Tukey test); DP of the EPSP slope and PS amplitude was 86±3% (EPSP) and 85±2% (PS) in control rats and 94±5% (EPSP) and 93±4% (PS) in congenitally infected rats (EPSP: F=5.62, P<0.05;PS: F=4.22, P<0.05, two-way ANOVA with Tukey test) . At the same time, intracellular [Ca(2+)] and mRNA level of CaM in the hippocampus neurons of the experiment group were significantly increased than that of in the controls ([Ca(2+)]: P<0.01;CaM mRNA: P<0.01) . The results demonstrate that congenital HCMV infection could reduce the range of synaptic plasticity in the Sprague-Dawley rats, which may trigger the dysfunction of learning and memory through disrupting the calcium balance.
本研究旨在探讨先天性 HCMV 感染是否会影响 LTP/DP 的诱导和维持。通过建立先天性感染 HCMV 的 Sprague-Dawley 大鼠模型，在其子代大鼠（50-65 日龄）海马切片中记录场兴奋性突触后电位（EPSP），以研究先天性感染后海马齿状回（DG）区 LTP/DP 的变化。测量实验组（先天性感染 HCMV）和对照组海马神经元中的 Ca（2+）和钙调蛋白（CaM）mRNA 水平；实验组（感染 HCMV）的 EPSP 斜率 PS 幅度在 DG 区的输入/输出（I/O）曲线明显低于对照组（P<0.05）。对照组大鼠海马 DG 区 EPSP 斜率和 PS 幅度的 LTP 分别为 137±4%（EPSP）和 225±11%（PS），实验组大鼠分别为 115±9%（EPSP）和 163±7%（PS）（EPSP：F=25.29，P<0.05；PS：F=74.33，P<0.05，双向方差分析加 Tukey 检验）；对照组大鼠 EPSP 斜率和 PS 幅度的 DP 分别为 86±3%（EPSP）和 85±2%（PS），实验组大鼠分别为 94±5%（EPSP）和 93±4%（PS）（EPSP：F=5.62，P<0.05；PS：F=4.22，P<0.05，双向方差分析加 Tukey 检验）。同时，实验组海马神经元内的 [Ca（2+）]和 CaM mRNA 水平明显高于对照组（[Ca（2+）]：P<0.01；CaM mRNA：P<0.01）。结果表明，先天性 HCMV 感染可减少 Sprague-Dawley 大鼠突触可塑性范围，可能通过破坏钙平衡导致学习记忆功能障碍。
