Department of Biological Sciences, Evolutionary and Comparative Biology Division, University Federico II of Naples, Via Mezzocannone 8, 80134 Naples, Italy.
Environ Pollut. 2011 May;159(5):1101-7. doi: 10.1016/j.envpol.2011.02.017. Epub 2011 Mar 11.
Endocrine Disruptor Chemicals (EDCs) with estrogen-like properties i.e nonylphenol (NP) induce vitellogenin (VTG) synthesis in males of aquatic and semi-aquatic species. In the oviparous species VTG is a female-specific oestrogen dependent protein. Males are unable to synthesize VTG except after E2 treatment. This study aimed to verify if NP, administered via food and water, is able to induce the expression of VTG even in males of vertebrates with a terrestrial habitat such as the lizard Podarcis. By means of ICC, ISH, W/B and ELISA we demonstrated that NP induces the presence of VTG in the plasma and its expression in the liver. VTG, undetectable in untreated males, reaches the value of 4.34 μg/μl in the experimental ones. Expression analysis and ISH in the liver showed that an NP-polluted diet also elicits the expression of ERα in the liver which is known to be related to VTG synthesis in Podarcis.
具有雌激素样特性的内分泌干扰化学物质（EDCs），如壬基酚（NP），会诱导水生和半水生物种雄性个体合成卵黄蛋白原（VTG）。在卵生物种中，VTG 是一种雌性特异性的雌激素依赖蛋白。除了经过 E2 处理外，雄性个体无法合成 VTG。本研究旨在验证 NP 是否能够通过食物和水的摄入，诱导具有陆地栖息地的脊椎动物雄性个体（如蜥蜴 Podarcis）表达 VTG。通过 ICC、ISH、W/B 和 ELISA，我们证明 NP 能够诱导 VTG 在血浆中的存在及其在肝脏中的表达。在未处理的雄性个体中无法检测到 VTG，而在实验组中，VTG 的浓度达到了 4.34μg/μl。肝脏的表达分析和 ISH 表明，受 NP 污染的饮食也会在肝脏中引发 ERα 的表达，而 ERα 已知与 Podarcis 中 VTG 的合成有关。
