Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. North, PO Box 19024, D3-100, Seattle, WA 98109-1024, United States.
J Clin Virol. 2011 May;51(1):50-3. doi: 10.1016/j.jcv.2011.02.010. Epub 2011 Mar 11.
Although data documenting the severity and frequency of human parechovirus (HPeV) infections have been published, detection of HPeV is not routinely performed in most clinical virology laboratories.
To describe diagnostic yield, epidemiology and clinical characteristics of patients infected with HPeV during the first year using a new HPeV reverse transcription (RT)-PCR.
We introduced an HPeV RT-PCR for the routine testing of cerebrospinal fluid (CSF) and blood samples submitted to our clinical laboratory for detection of human enteroviruses (HEV). Prospective testing of samples with retrospective analysis of medical charts was performed.
Of the 499 clinical samples received between May, 2009 and May, 2010, 9.6% (46 patients) had HEV detected and 3.4% (15 patients) had HPeV detected. All patients infected by HPeV were <3 months old, hospitalized between June and October 2009, and all typed viruses were HPeV3. Clinical characteristics of HPeV and HEV infected infants were similar. However, patients infected with HPeV were more likely to have a normal leukocyte count in their CSF (p<0.001). One HPeV3-infected infant developed encephalitis and another developed hepatitis.
In our institution, the HPeV RT-PCR was useful to diagnose a novel pathogen in infants with sepsis-like disease.
虽然已有文献记录了人类肠道病毒（HPeV）感染的严重程度和频率，但大多数临床病毒学实验室并未常规检测 HPeV。
使用新的 HPeV 逆转录（RT）-PCR 描述第一年感染 HPeV 的患者的诊断率、流行病学和临床特征。
我们引入了一种 HPeV RT-PCR，用于常规检测提交给我们临床实验室以检测人类肠道病毒（HEV）的脑脊液（CSF）和血液样本。对样本进行前瞻性检测，并对病历进行回顾性分析。
在 2009 年 5 月至 2010 年 5 月期间收到的 499 份临床样本中，9.6%（46 例患者）检测到 HEV，3.4%（15 例患者）检测到 HPeV。所有感染 HPeV 的患者均<3 个月大，住院时间为 2009 年 6 月至 10 月，所有分型病毒均为 HPeV3。感染 HPeV 和 HEV 的婴儿的临床特征相似。然而，感染 HPeV 的患者脑脊液白细胞计数更可能正常（p<0.001）。1 例 HPeV3 感染婴儿发生脑炎，另 1 例发生肝炎。
在我们的机构中，HPeV RT-PCR 可用于诊断类似败血症疾病的婴儿中的新型病原体。
