Case Western Reserve University, Cleveland, OH 44106, USA.
Vaccine. 2011 Apr 27;29(19):3558-63. doi: 10.1016/j.vaccine.2011.02.092. Epub 2011 Mar 11.
We evaluated immunologic predictors of response to HBV vaccine administered in the presence or absence of GM-CSF in HIV infected individuals. We measured peripheral blood hematopoietic progenitor, monocyte and myeloid-derived suppressor cell (MDSC) frequencies, and expression of GMCSF receptor on monocytes and MDSCs, at baseline and 4weeks after immunization in relation to antibody response. We observed higher baseline progenitor and lower monocyte frequencies among week 16 antibody responders. Week 4 decline in MDSC frequency was associated with week 16 antibody response, while administration of GM-CSF was associated with preservation of these cells. No significant differences in GM-CSF receptor expression were observed in the presence vs. absence of GM-CSF. These findings are consistent with a positive role of progenitor cells and a potential negative role of monocytes in vaccine response. Additionally, GM-CSF augmented the preservation of peripheral blood MDSC, which may contribute to the lack of improved vaccine responses.
我们评估了在 HIV 感染者中存在或不存在 GM-CSF 的情况下接种乙肝疫苗的免疫预测因子与抗体反应的关系。我们测量了外周血造血祖细胞、单核细胞和髓源性抑制细胞（MDSC）的频率，以及 GM-CSF 受体在单核细胞和 MDSC 上的表达，在免疫接种前后 4 周与抗体反应相关。我们观察到，在第 16 周抗体反应者中，祖细胞的基线频率更高，单核细胞的频率更低。MDSC 频率在第 4 周下降与第 16 周抗体反应相关，而 GM-CSF 的给药与这些细胞的保留相关。在存在或不存在 GM-CSF 的情况下，GM-CSF 受体的表达没有明显差异。这些发现与祖细胞在疫苗反应中的积极作用和单核细胞在疫苗反应中的潜在消极作用一致。此外，GM-CSF 增强了外周血 MDSC 的保留，这可能导致疫苗反应没有得到改善。
