Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Z.P. 11340, México, D.F., Mexico.
Immunobiology. 2011 Aug;216(8):925-35. doi: 10.1016/j.imbio.2011.01.004. Epub 2011 Jan 12.
Endothelial cells are susceptible to infection by several pathogens, but little is known about mycobacterial infection. We analyzed some features of mycobacteria-endothelial cell interactions and the innate response to the infection. Intracellular growth in human umbilical vein endothelial cells (HUVECs) of three Mycobacterium species: M. tuberculosis (MTB), M. abscessus (MAB) and M. smegmatis (MSM) was analyzed. M. smegmatis was eliminated; M. abscessus had an accelerate intracellular replication and M. tuberculosis did not replicate or was eliminated. M. abscessus infection induced profound cytoskeleton rearrangements, with M. tuberculosis infection changes were less marked, and with MSM were slight. Nitric oxide (NO) production was induced differentially: M. abscessus induced the highest levels followed by M. tuberculosis and M. smegmatis; the contrary was true for reactive oxygen species (ROS) production. Only M. tuberculosis infection caused beta-1 defensin over-expression. As a whole, our results describe some aspects of the innate response of HUVEC infected by mycobacteria with different virulence and suggest that a strong cytoskeleton mobilization triggers a high NO production in these cells.
内皮细胞容易受到几种病原体的感染，但对分枝杆菌感染知之甚少。我们分析了分枝杆菌-内皮细胞相互作用的一些特征和对感染的固有反应。分析了三种分枝杆菌物种（结核分枝杆菌（MTB）、脓肿分枝杆菌（MAB）和耻垢分枝杆菌（MSM））在人脐静脉内皮细胞（HUVEC）中的细胞内生长。耻垢分枝杆菌被消除；脓肿分枝杆菌具有加速的细胞内复制能力，而结核分枝杆菌既不复制也不被消除。脓肿分枝杆菌感染诱导了深刻的细胞骨架重排，而结核分枝杆菌感染的变化不那么明显，而耻垢分枝杆菌感染的变化则很轻微。一氧化氮（NO）的产生被不同程度地诱导：脓肿分枝杆菌诱导的水平最高，其次是结核分枝杆菌和耻垢分枝杆菌；而活性氧（ROS）的产生则相反。只有结核分枝杆菌感染导致β-1 防御素过度表达。总的来说，我们的结果描述了不同毒力的分枝杆菌感染 HUVEC 时固有反应的一些方面，并表明强烈的细胞骨架动员会在这些细胞中引发高水平的 NO 产生。
