Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea.
Mol Cells. 2011 May;31(5):447-54. doi: 10.1007/s10059-011-0300-7. Epub 2011 Mar 9.
We carried out activation tagging screen to isolate genes regulating abscisic acid (ABA) response. From the screen of approximately 10,000 plants, we isolated ca 100 ABA response mutants. We characterized one of the mutants, designated ahs1, in this study. The mutant is ABA-hypersensitive, and AtMYB52 was found to be activated in the mutant. Overexpression analysis to recapitulate the mutant phenotypes demonstrated that ATMYB confers ABA-hypersensitivity during postgermination growth. Additionally, AtMYB52 overexpression lines were drought-tolerant and their seedlings were salt-sensitive. Changes in the expression levels of a few genes involved in ABA response or cell wall biosynthesis were also observed. Together, our data suggest that AtMYB52 is involved in ABA response. Others previously demonstrated that AtMYB52 regulates cell wall biosynthesis; thus, our results imply a possible connection between ABA response and cell wall biosynthesis.
我们进行了激活标签筛选，以分离调节脱落酸（ABA）反应的基因。在大约 10000 株植物的筛选中，我们分离出约 100 个 ABA 反应突变体。在本研究中，我们对其中一个突变体，命名为 ahs1，进行了特征描述。该突变体对 ABA 敏感，并且在突变体中发现 AtMYB52 被激活。为了重现突变体表型的过表达分析表明，ATMYB 在萌发后生长过程中赋予 ABA 敏感性。此外，AtMYB52 过表达系耐旱，其幼苗对盐敏感。还观察到参与 ABA 反应或细胞壁生物合成的少数基因的表达水平发生变化。总之，我们的数据表明 AtMYB52 参与 ABA 反应。其他人先前证明 AtMYB52 调节细胞壁生物合成；因此，我们的结果暗示 ABA 反应和细胞壁生物合成之间可能存在联系。
