Laboratory of Botany, Anhui Wenda University of Information Engineering, Hefei, Anhui, China.
School of Life Sciences, Anhui Agricultural University, Hefei, Anhui, China.
PeerJ. 2024 Apr 25;12:e17304. doi: 10.7717/peerj.17304. eCollection 2024.
The gene family exerts significant influence over various biological processes and stress responses in plants. Despite this, a comprehensive analysis of this gene family in pumpkin remains absent. In this study, the genes of were identified and clustered into 33 groups (C1-33), with members of each group being highly conserved in terms of their motif composition. Furthermore, the distribution of 175 genes across all 20 chromosomes was found to be non-uniform. Examination of the promoter regions of these genes revealed the presence of cis-acting elements associated with phytohormone responses and abiotic/biotic stress. Utilizing quantitative real-time polymerase chain reaction (qRT-PCR), the expression patterns of 13 selected genes were validated, particularly in response to exogenous phytohormone exposure and various abiotic stressors, including ABA, SA, MeJA, and drought treatments. Expression analysis in different tissues showed that genes are expressed at different levels in different tissues, suggesting that they are functionally divergent in regulating growth and abiotic stresses. These results provide a basis for future studies to characterize the function of the gene family under abiotic stresses in pumpkins.
该基因家族对植物的各种生物过程和应激反应有重要影响。尽管如此，对南瓜中这个基因家族的综合分析仍然缺失。在这项研究中，鉴定了 的基因，并将它们聚类为 33 组（C1-33），每组的成员在基序组成方面高度保守。此外，发现 175 个基因在所有 20 条染色体上的分布不均匀。对这些基因启动子区域的研究表明，存在与植物激素反应和非生物/生物胁迫相关的顺式作用元件。利用定量实时聚合酶链反应（qRT-PCR），验证了 13 个选定的 基因的表达模式，特别是对外源植物激素暴露和各种非生物胁迫因子（如 ABA、SA、MeJA 和干旱处理）的响应。在不同组织中的表达分析表明， 基因在不同组织中以不同的水平表达，表明它们在调节生长和非生物胁迫方面的功能是不同的。这些结果为未来研究在南瓜中非生物胁迫下 基因家族的功能提供了基础。
