Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic.
Physiol Res. 2011;60(3):503-10. doi: 10.33549/physiolres.931969. Epub 2011 Mar 14.
It would be desirable to expand the existing general knowledge concerning direct action of metals on the ovary. Nevertheless, the results of testing of iron compound on porcine ovarian cells should be interpreted carefully because iron is an essential element which could also induce changes in cellular processes. The aim of this in vitro study was 1) to examine dose-dependent effects of iron on the secretory activity of porcine ovarian granulosa cells, and 2) to outline the potential intracellular mediators mediating these effects. Specifically, we evaluated the effect of iron sulphate on the release of insulin-like growth factor I (IGF-I) and progesterone, as well as the expression of markers of proliferation (cyclin B1) and apoptosis (caspase-3) in porcine ovarian granulosa cells. Concentrations of IGF-I and progesterone were determined by RIA, cyclin B1 and caspase-3 expression by immunocytochemistry (ICC). Our results show a significantly decreased IGF-I secretion by ovarian granulosa cells after iron sulphate addition at the doses 0.5 and 1.0 mg/ml. The iron sulphate additions at doses 0.17 and 1.0 mg/ml had no effect on progesterone secretion. In contrast, iron sulphate addition at doses 0.17-1.0 mg/ml resulted in stimulation of cyclin B1 and caspase-3 expression. In conclusion, the present results indicate a direct effect of iron on 1) secretion of growth factor IGF-I but not steroid hormone progesterone, 2) expression of markers of proliferation (cyclin B1), or 3) apoptosis (caspase-3) of porcine ovarian granulosa cells. These results support an idea that iron could play a regulatory role in porcine ovarian function: hormone release, proliferation and apoptosis.
希望能够扩展关于金属对卵巢直接作用的现有一般知识。然而，由于铁是一种必需元素，也可能诱导细胞过程发生变化，因此应该谨慎解释铁化合物对猪卵巢细胞的测试结果。本体外研究的目的是：1）检验铁对猪卵巢颗粒细胞分泌活性的剂量依赖性影响，2）概述介导这些影响的潜在细胞内介质。具体而言，我们评估了硫酸铁对胰岛素样生长因子 I（IGF-I）和孕酮释放的影响，以及猪卵巢颗粒细胞中增殖标志物（细胞周期蛋白 B1）和凋亡标志物（半胱天冬酶-3）的表达。通过放射免疫分析（RIA）测定 IGF-I 和孕酮的浓度，通过免疫细胞化学（ICC）测定细胞周期蛋白 B1 和半胱天冬酶-3的表达。我们的结果表明，硫酸铁添加剂量为 0.5 和 1.0 mg/ml 时，猪卵巢颗粒细胞分泌的 IGF-I 显著减少。硫酸铁添加剂量为 0.17 和 1.0 mg/ml 时对孕酮分泌没有影响。相反，硫酸铁添加剂量为 0.17-1.0 mg/ml 时导致细胞周期蛋白 B1 和半胱天冬酶-3表达增加。总之，本研究结果表明铁对 1）生长因子 IGF-I 的分泌有直接作用，但对类固醇激素孕酮的分泌没有作用，2）猪卵巢颗粒细胞的增殖标志物（细胞周期蛋白 B1）的表达或 3）凋亡（半胱天冬酶-3）有直接作用。这些结果支持铁可能在猪卵巢功能中发挥调节作用的观点：激素释放、增殖和凋亡。
