Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2010;45(3):320-31. doi: 10.1080/10934520903467907.
The heavy metals lead (Pb) and mercury (Hg) pose potential risks to sustainability of environment and thus to our future generations. General objective of this in vitro study was to examine the secretory activity of porcine ovarian granulosa cells after Pb and Hg administration and to outline the potential intracellular mediators of its effects. For this purpose, release of insulin-like growth factor I (IGF-I) and steroid hormone progesterone (P(4)), expression of proliferation- related (cyclin B1) and apoptosis-related (caspase-3) peptides was examined in porcine ovarian granulosa cells after heavy metals administration. Obtained data indicate Pb-induced inhibition of IGF-I release at lower doses (0.063 mg/mL and 0.046 mg/mL) by ovarian granulosa cells. However, P(4) release was not influenced by Pb addition, while the expression of cyclin B1 and caspase-3 was induced by Pb addition. These results indicate that Pb can affect the pathway of proliferation and apoptosis of porcine ovarian granulosa cells through intracellular substances such as cyclin B1 and caspase-3. On the other hand, the P(4) release by ovarian granulosa cells of pregnant gilts was stimulated by experimental Pb administration at doses of 0.25 mg/mL and 0.063 mg/mL and experimental Hg administration at doses 0.25 mg/mL and 0.083 mg/mL. P(4) release by ovarian cells of pregnant gilts was not influenced by a combinatory dose of FSH (1.0 ng/mL) + Pb (0.083 mg/mL) + Hg (0.083 mg/mL) but it was inhibited by experimental administration of FSH (10 ng/mL) + Pb (0.25 ng/mL) + Hg (0.25 ng/mL). Possible involvement of heavy metals - Pb and Hg and pituitary hormone FSH, in the regulation of P(4) release by porcine ovarian granulosa cells of pregnant gilts was noted. Data obtained from in vitro studies suggest the dose dependent association of heavy metals administration with the hormonal release by porcine ovarian granulosa cells. This association also depended on pregnancy of the gilts.
重金属铅（Pb）和汞（Hg）对环境的可持续性构成潜在风险，从而对我们的后代构成潜在风险。本体外研究的总体目标是研究 Pb 和 Hg 给药后猪卵巢颗粒细胞的分泌活性，并概述其作用的潜在细胞内介质。为此，在 Pb 和 Hg 给药后，检查了猪卵巢颗粒细胞中胰岛素样生长因子 I（IGF-I）和类固醇激素孕酮（P(4)）的释放以及与增殖相关的（细胞周期蛋白 B1）和凋亡相关的（半胱天冬酶-3）肽的表达。获得的数据表明，较低剂量（0.063 mg/mL 和 0.046 mg/mL）的 Pb 会抑制卵巢颗粒细胞中 IGF-I 的释放。然而，P(4)的释放不受 Pb 加入的影响，而细胞周期蛋白 B1 和半胱天冬酶-3的表达则受 Pb 加入的诱导。这些结果表明，Pb 可以通过细胞内物质（如细胞周期蛋白 B1 和半胱天冬酶-3）影响猪卵巢颗粒细胞的增殖和凋亡途径。另一方面，实验性 Pb 给药（剂量为 0.25 mg/mL 和 0.063 mg/mL）和实验性 Hg 给药（剂量为 0.25 mg/mL 和 0.083 mg/mL）可刺激怀孕母猪卵巢颗粒细胞的 P(4)释放。由 FSH（1.0 ng/mL）+Pb（0.083 mg/mL）+Hg（0.083 mg/mL）联合剂量给药不影响卵巢细胞的 P(4)释放，但实验性 FSH（10 ng/mL）+Pb（0.25 ng/mL）+Hg（0.25 ng/mL）给药抑制 P(4)释放。注意到重金属-Pb 和 Hg 以及垂体激素 FSH 可能参与调节怀孕母猪卵巢颗粒细胞的 P(4)释放。体外研究获得的数据表明，重金属给药与猪卵巢颗粒细胞激素释放之间存在剂量依赖性关联。这种关联还取决于母猪的怀孕情况。
