Department of Animal Physiology, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
J Environ Sci Health B. 2011;46(3):213-9. doi: 10.1080/03601234.2011.540205.
The aim of this in vitro study was to examine the secretion activity, markers of proliferation and apoptosis in porcine ovarian granulosa cells (GCs) after deoxynivalenol (DON) addition. Ovarian granulosa cells were incubated with DON for 24h: 10, 100 and 1000 ng/mL, while the control group received no DON. The secretion of insulin-like growth factor I (IGF-I) and progesterone was determined by radioimmunoassay (RIA) and expression of cyclin B1, PCNA and caspase-3 by immunocytochemistry. IGF-I release by GCs was inhibited by DON, while progesterone release and the expression of cyclin B1 was stimulated by DON (at 1000 ng/mL but not at 10 and 100 ng/mL). PCNA expression was stimulated by DON (at 100 and 1000 ng/mL but not at 10 ng/mL). Caspase-3 expression was not influenced by DON treatment (at all doses). In conclusion, our results indicate, (1) a direct effect of DON on secretion of growth factor IGF-I and steroid hormone progesterone, (2) expression of markers of proliferation (cyclin B1 and PCNA) but not on the (3) expression of marker of apoptosis (caspase-3) in porcine ovarian granulosa cells. This in vitro study suggests the dose-dependent association of DON on porcine ovarian functions.
本体外研究旨在检测脱氧雪腐镰刀菌烯醇（DON）作用后猪卵巢颗粒细胞（GCs）的分泌活性、增殖和凋亡标志物。将卵巢颗粒细胞与 DON 孵育 24 小时：10、100 和 1000ng/ml，而对照组未添加 DON。通过放射免疫测定（RIA）检测胰岛素样生长因子 I（IGF-I）和孕激素的分泌，通过免疫细胞化学检测细胞周期蛋白 B1、PCNA 和半胱天冬酶-3 的表达。DON 抑制 GCs 的 IGF-I 释放，而 DON 刺激孕激素释放和细胞周期蛋白 B1 的表达（在 1000ng/ml 时，但在 10 和 100ng/ml 时没有）。PCNA 表达受 DON 刺激（在 100 和 1000ng/ml 时，但在 10ng/ml 时没有）。DON 处理对 caspase-3 表达没有影响（在所有剂量下）。总之，我们的结果表明，（1）DON 对生长因子 IGF-I 和甾体激素孕激素分泌的直接影响，（2）增殖标志物（细胞周期蛋白 B1 和 PCNA）的表达，但不影响（3）猪卵巢颗粒细胞中凋亡标志物（caspase-3）的表达。这项体外研究表明 DON 与猪卵巢功能之间存在剂量依赖性关联。
