China National Cosmetics Quality Supervision & Inspection Center, Beijing 100094, China.
Int J Cosmet Sci. 2011 Oct;33(5):408-15. doi: 10.1111/j.1468-2494.2011.00643.x. Epub 2011 Mar 15.
A method of gradient elution high-performance liquid chromatography (HPLC) for simultaneous determination of 11 different ultraviolet-absorbing chemicals of phenylbenzlmldazole sulphonic acid, 4-aminobenzoic acid, benzophenone-4, benzophenone-3, isoamyl p-methoxycinnamate, 4-methylbenzylidene camphor, octocrylene, ethylhexyl methoxycinnamate, homosalate, ethylhexyl salicylate, methylene bis-benzotriazolyl tetramethylbutyl phenol was developed for the application to sunscreen cosmetic products. In this study, an Agilent SB-C18 analytical column (250 × 4.6 mm, 5 μm) was utilized and methanol, tetrahydrofuran and perchloric acid aqueous solution (0.2 mL HClO(4) + 300 mL H(2)O) were used for gradient elution at a total flow rate of 1.0 mL min(-1). The optimum conditions for 11 different ultraviolet-absorbing chemicals analyses were investigated. All calibration curves showed good linear regression with UV detection (311 nm) within test ranges. The correlation coefficients were better than 0.999 in all cases. The assay was simple, selective, convenient and reproducible and is suitable for the determination of ultraviolet-absorbing chemicals in commercial sunscreen cosmetic products. The use frequency of 11 different ultraviolet absorbents in 100 sunscreen cosmetics was investigated and statistically analysed. The ultraviolet absorbent of maximum use frequency was ethylhexyl methoxycinnamate.
一种梯度洗脱高效液相色谱（HPLC）方法，用于同时测定苯并咪唑磺酸、4-氨基苯甲酸、二苯甲酮-4、二苯甲酮-3、异戊基对甲氧基肉桂酸酯、4-甲基苄叉基樟脑、奥克立林、乙基己基甲氧基肉桂酸酯、胡莫柳酯、水杨酸乙基己酯、亚甲基双-苯并三唑基四甲基丁基酚等 11 种不同的紫外吸收化学物质。本方法用于防晒化妆品。在本研究中，采用 Agilent SB-C18 分析柱（250×4.6mm，5μm），甲醇、四氢呋喃和高氯酸水溶液（0.2mL HClO(4)+300mL H(2)O）在总流速为 1.0mL/min 的条件下进行梯度洗脱。考察了 11 种不同紫外吸收化学物质分析的最佳条件。所有校准曲线在测试范围内均具有良好的紫外检测（311nm）线性回归，相关系数均大于 0.999。该测定方法简单、选择性好、方便、重现性好，适用于测定商业防晒化妆品中的紫外吸收化学物质。调查并统计分析了 100 种防晒化妆品中 11 种不同紫外线吸收剂的使用频率。使用频率最高的紫外线吸收剂为乙基己基甲氧基肉桂酸酯。
