Sobańska Anna W, Kałębasiak Katarzyna, Pyzowski Jarosław, Brzezińska Elżbieta
Department of Analytical Chemistry, Medical University of Lodz, Ulica Muszynskiego 1, 90-151 Lodz, Poland.
J Anal Methods Chem. 2015;2015:695658. doi: 10.1155/2015/695658. Epub 2015 Feb 4.
Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of chromatograms was performed at 285 nm. The densitometric calibration curve for BZ4 was nonlinear (second-degree polynomial), with R > 0.999. The limits of detection and quantification were ca. 0.03 and ca. 0.1 μg spot(-1), respectively. The results obtained by HPTLC-densitometry were compared to those obtained by zero and 2nd derivative UV spectrophotometry. In the case of spectrophotometric methods, calibration curves were linear with R > 0.9998. The chromatographic method was fully validated.
通过在硅胶60固定相上进行薄层色谱法，以乙酸乙酯 - 乙醇 - 水 - pH 6磷酸盐缓冲液（15∶7∶5∶1，v/v/v/v）作为流动相，从洗发水的表面活性剂、染料、防腐剂和其他成分中分离出二苯甲酮 - 4（BZ4）。在285 nm处对色谱图进行光密度扫描。BZ4的光密度校准曲线为非线性（二次多项式），R>0.999。检测限和定量限分别约为0.03和约0.1μg斑点⁻¹。将高效薄层色谱 - 光密度法获得的结果与零阶和二阶导数紫外分光光度法获得的结果进行比较。在分光光度法的情况下，校准曲线呈线性，R>0.9998。该色谱方法得到了充分验证。
