一种用于免疫荧光染色 CD133 阳性脑胶质瘤干细胞的新型固定剂。
A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells.
机构信息
Department of Neurological Surgery, University of Virginia, Charlottesville, Virginia, USA.
出版信息
J Neurosci Methods. 2011 May 15;198(1):99-102. doi: 10.1016/j.jneumeth.2011.03.003. Epub 2011 Mar 23.
Abstract
Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1,4-benzoquinone) for immunoflourescence staining of neurospheres.
摘要
胶质母细胞瘤干细胞的分离需要将肿瘤细胞在神经干细胞培养基中孵育。大约五天后，含有这些胶质母细胞瘤干细胞的神经球就会形成。然后可以分析这些细胞中干细胞标志物的存在情况。这些标志物的免疫荧光染色可以作为一种有价值的工具，用于直接在干细胞培养基中分析完整的神经球。在这里，我们介绍了一种新型固定剂（1,4-苯醌）在神经球免疫荧光染色中的应用。
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