Institute of Nutrition, Department of Nutritional Toxicology, Friedrich Schiller University Jena, Dornburger Straße 24, 07743 Jena, Germany.
Biochimie. 2011 Jun;93(6):1076-9. doi: 10.1016/j.biochi.2011.03.004. Epub 2011 Mar 21.
A substantial part of soluble, oxidized proteins are degraded by the proteasome. However, it is still under debate whether these oxidized proteins are degraded by the 26S proteasome in an ubiquitin-dependent way or in an ubiquitin-independent way by the 20S proteasome. Therefore, we treated cells with H(2)O(2) and UV-A irradiation and detected protein carbonyls and ubiquitination by immunoblotting. Separation of ubiquitinated proteins from non-ubiquitinated reveals that most oxidized proteins are not ubiquitinated.
大量可溶的、氧化的蛋白质被蛋白酶体降解。然而，这些氧化的蛋白质是否通过泛素依赖性方式被 26S 蛋白酶体降解，或者通过非泛素依赖性方式被 20S 蛋白酶体降解，这仍存在争议。因此，我们用 H2O2 和 UV-A 照射处理细胞，并通过免疫印迹检测蛋白质羰基和泛素化。将泛素化的蛋白质与非泛素化的蛋白质分离表明，大多数氧化的蛋白质没有被泛素化。
