Sosnowski Tomasz R, Koliński Michal, Gradón Leon
Faculty of Chemical and Process Engineering, Warsaw University of Technology, 00-645 Warsaw, Poland.
Ann Occup Hyg. 2011 Apr;55(3):329-38. doi: 10.1093/annhyg/mer007.
One of the reasons for cellular changes in the lung tissue exposed to the diesel exhaust composed of soot particles with adsorbed volatile organic molecules is the reduction of the clearance rate in the pulmonary region of the respiratory system. The interaction of the fractal-like particles and organic substances with a surfactant monolayer limits its dynamic activity. The surface properties of Survanta, a purified extract of bovine lung surfactant (LS), which interacted with carbon particles (200 nm aggregates) and benzo[a]pyrene (BaP), molecules were measured with the oscillating bubble technique. The results showed a significant lowering of the dynamics of the surfactant monolayer compared to the control case (no exposure). Additional measurements of surface pressure during the monotonic compression of the air-water interface containing the major LS phospholipid, dipalmitoylphosphatidylcholine (DPPC), showed that the presence of BaP molecules in the system influenced its stability. The experimental results were supplemented with a theoretical molecular dynamics model of the interaction between BaP and DPPC molecules. The simulation results indicated the insertion of BaP molecules into the lipid layer, which explained the measured effects.
暴露于由吸附了挥发性有机分子的烟尘颗粒组成的柴油废气中的肺组织发生细胞变化的原因之一，是呼吸系统肺部区域清除率的降低。类分形颗粒和有机物质与表面活性剂单分子层的相互作用限制了其动态活性。采用振荡气泡技术测量了与碳颗粒（200nm聚集体）和苯并[a]芘（BaP）分子相互作用的牛肺表面活性剂（LS）纯化提取物Survanta的表面性质。结果表明，与对照情况（未暴露）相比，表面活性剂单分子层的动力学显著降低。对含有主要LS磷脂二棕榈酰磷脂酰胆碱（DPPC）的气-水界面进行单调压缩时的表面压力的额外测量表明，系统中BaP分子的存在影响了其稳定性。实验结果辅以BaP与DPPC分子相互作用的理论分子动力学模型。模拟结果表明BaP分子插入脂质层，这解释了所测得的效应。
