Iordan Andreea, Duperray Alain, Gérard Anaïs, Grichine Alexeï, Verdier Claude
Laboratoire de Spectrométrie Physique, CNRS-Université Grenoble I, 140 Avenue de la Physique, Saint-Martin d’Hères cedex, France.
Biorheology. 2010;47(5-6):277-95. doi: 10.3233/BIR-2010-0575.
Collagen model tissues, consisting of cells embedded in a collagen matrix at different concentrations (of cells and collagen) were analyzed. Rheological properties were measured and complementary confocal microscopy analysis carried out. An important feature, corresponding to the breakdown of the collagen network (i.e., decrease in network elasticity) was observed at high collagen concentrations, due to the presence of cells. Thanks to confocal microscopy, we showed that cells elongated within the gel and could remodel it, this being a concentration-dependent feature. A careful analysis of the remodeling process showed that cells can attract collagen in their close neighborhood, this being an irreversible process and that migrating cells create collagen-depleted regions behind them.
对由嵌入不同浓度（细胞和胶原蛋白）胶原蛋白基质中的细胞组成的胶原蛋白模型组织进行了分析。测量了流变学特性并进行了补充共聚焦显微镜分析。在高胶原蛋白浓度下，由于细胞的存在，观察到了与胶原蛋白网络破坏相对应的一个重要特征（即网络弹性降低）。借助共聚焦显微镜，我们发现细胞在凝胶内伸长并能够对其进行重塑，这是一个浓度依赖性特征。对重塑过程的仔细分析表明，细胞可以在其紧邻区域吸引胶原蛋白，这是一个不可逆的过程，并且迁移的细胞在它们后面形成胶原蛋白耗尽区域。
