Unit of Clinical Microbiology, Department of Surgical Science, Faculty of Medicine, University of Messina, 98125 Messina, Italy.
Mediators Inflamm. 2010;2010:613937. doi: 10.1155/2010/613937. Epub 2011 Mar 3.
NGAL is involved in modulation of the inflammatory response and is found in the sera of uremic patients. We investigated whether hemodiafiltration (HDF) could influence the ability of polymorphonuclear granulocytes (PMGs) to release NGAL. The involvement of interleukin- (IL-)1β and tumor necrosis factor- (TNF-)α on NGAL release was evaluated.
We studied end-stage renal disease (ESRD) patients at the start of dialysis (Pre-HDF) and at the end of treatment (Post-HDF) and 18 healthy subjects (HSs). Peripheral venous blood was taken from HDF patients at the start of dialysis and at the end of treatment.
PMGs obtained from ESRD patients were hyporesponsive to LPS treatment, with respect to PMG from HS. IL-1β and TNF-α produced by PMG from post-HDF patients were higher than those obtained by PMG from pre-HDF. Neutralization of IL-1β, but not of TNF-α, determined a clear-cut production of NGAL in PMG from healthy donors. On the contrary, specific induction of NGAL in PMG from uremic patients was dependent on the presence in supernatants of IL-1β and TNF-α.
Our data demonstrate that in PMG from healthy subjects, NGAL production was supported solely by IL-1β, whereas in PMG from HDF patients, NGAL production was supported by IL-1β, TNF-α.
中性粒细胞明胶酶相关脂质运载蛋白（NGAL）参与炎症反应的调节，在尿毒症患者的血清中被发现。我们研究了血液透析滤过（HDF）是否会影响多形核粒细胞（PMG）释放 NGAL 的能力。评估了白细胞介素-（IL-）1β和肿瘤坏死因子-（TNF-）α对 NGAL 释放的影响。
我们研究了开始透析时的终末期肾病（ESRD）患者（HDF 前）和治疗结束时（HDF 后）以及 18 名健康受试者（HS）。从 HDF 患者开始透析和治疗结束时采集外周静脉血。
与 HS 的 PMG 相比，ESRD 患者的 PMG 对 LPS 治疗的反应性降低。来自 HDF 后患者的 PMG 产生的 IL-1β 和 TNF-α高于来自 HDF 前患者的 PMG。中和 IL-1β，但不中和 TNF-α，可导致健康供体 PMG 中 NGAL 的明显产生。相反，尿毒症患者 PMG 中 NGAL 的特异性诱导取决于 IL-1β 和 TNF-α在上清液中的存在。
我们的数据表明，在健康受试者的 PMG 中，NGAL 的产生仅由 IL-1β 支持，而在 HDF 患者的 PMG 中，IL-1β 和 TNF-α支持 NGAL 的产生。
