Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Sep Sci. 2011 May;34(9):1004-10. doi: 10.1002/jssc.201000921. Epub 2011 Mar 15.
A simple and reliable headspace GC-flame ionization detection (HS-GC-FID) method has been developed and validated for the simultaneous determination of seven volatile compounds of forensic interest: sevoflurane, desflurane, ethanol, methanol, 1-propanol, acetone and acetaldehyde. All seven compounds including acetonitrile (internal standard) eluted within 10 min and were well resolved with no endogenous interference. Good linearity was observed in the range of 1-12 mg/dL for both anesthetics and 2.5-40 mg/dL for the other five analytes. The method showed good precision, sensitivity and repeatability. Most of the analytes remained stable during the storage of samples at 4°C. Desflurane and acetone degraded (>10%), when the samples remained on the autosampler for more than 2 and 3 h, respectively. The method was finally applied on clinical and post-mortem blood and urine samples. The clinical samples were collected both from patients who underwent surgery, as well as from the occupationally exposed medical and nursing staff of the university hospital, working in the operating rooms. The hospital staff samples were found negative for all compounds, while the patients' samples were found positive for the anesthetic administered to the patient. The post-mortem blood samples were found positive for ethanol and acetaldehyde.
已开发并验证了一种简单可靠的顶空 GC-火焰离子化检测 (HS-GC-FID) 方法，用于同时测定七种具有法医学意义的挥发性化合物：七氟醚、地氟醚、乙醇、甲醇、1-丙醇、丙酮和乙醛。所有七种化合物（包括内标乙腈）均在 10 min 内洗脱，且无内源性干扰，均得到良好分离。两种麻醉剂在 1-12 mg/dL 范围内，其他五种分析物在 2.5-40 mg/dL 范围内均表现出良好的线性。该方法具有良好的精密度、灵敏度和重现性。大多数分析物在 4°C 下储存时稳定。当样品在自动进样器上放置超过 2 小时和 3 小时时，地氟醚和丙酮分别降解（>10%）。该方法最终应用于临床和死后血液和尿液样本。临床样本分别采集于接受手术的患者以及大学医院手术室工作的职业暴露的医护人员。医院工作人员样本中所有化合物均为阴性，而患者样本中则检测到给予患者的麻醉剂。死后血液样本中检测到乙醇和乙醛。
