Department of Plant Sciences and Entomology, University of Rhode Island, Kingston, RI 02881, USA.
J Econ Entomol. 2011 Feb;104(1):41-6. doi: 10.1603/EC10210.
Bacillus thuringiensis subspecies kurstaki and aizawai are important control agents for lepidopteran pests. Bioassays were designed to test B. t. kurstaki and aizawai against second- and-fourth instar black cutworm larvae with and without Bacillus sp. NFD2 and Pseudomonas sp. FNFD1 bacteria. B. thuringiensis subsp. aizawai (XenTari) was more toxic to both second- and fourth-instar black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), larvae than B. t. kurstaki (DiPel) at 7 d after treatment (DAT). When DiPel was combined with NFD2 or FNFD1 versus second instars, the LC50s were 5.0X and 4.7X lower, respectively, than with DiPel alone. DiPel combined with both NFD2 and FNFD1 versus second instars resulted in an LC50 value 7.7X lower than with DiPel alone. When XenTari was combined with NFD2 or FNFD1 versus second instars, the LC50s were 5.2X and 3.8X lower, respectively, than with XenTari alone. XenTari combined with both NFD2 and FNFD1 versus second instars resulted in an LC50 9.7X lower than with XenTari alone. When DiPel was combined with NFD2 or FNFD1 versus fourth instars, the LC50s were 4.4X and 3.4X lower, respectively, than with DiPel alone. DiPel combined with both NFD2 and FNFD1 versus fourth instars resulted in an LC50 5.0X lower than with DiPel alone. When XenTari was combined with NFD2 or FNFD1 versus fourth instars, the LC50s were 5.7X and 3.3X lower, respectively, than with XenTari alone. XenTari combined with both NFD2 and FNFD1 versus fourth instars resulted in an LC50 6.7X lower than with XenTari alone.
苏云金芽孢杆菌亚种 kurstaki 和 aizawai 是鳞翅目害虫的重要控制剂。设计生物测定法以测试含有和不含有芽孢杆菌 NFD2 和假单胞菌 FNFD1 细菌的二龄和四龄甜菜夜蛾幼虫对 B. t. kurstaki 和 aizawai。亚种 aizawai (XenTari) 对二龄和四龄甜菜夜蛾幼虫，Agrotis ipsilon (Hufnagel)（鳞翅目：夜蛾科）的毒力均高于 B. t. kurstaki (DiPel)，处理后 7 天（DAT）。当 DiPel 与 NFD2 或 FNFD1 联合使用时，二龄幼虫的 LC50 分别比单独使用 DiPel 低 5.0X 和 4.7X。DiPel 与 NFD2 和 FNFD1 联合使用时，二龄幼虫的 LC50 值比单独使用 DiPel 低 7.7X。当 XenTari 与 NFD2 或 FNFD1 联合使用时，二龄幼虫的 LC50 分别比单独使用 XenTari 低 5.2X 和 3.8X。XenTari 与 NFD2 和 FNFD1 联合使用时，二龄幼虫的 LC50 值比单独使用 XenTari 低 9.7X。当 DiPel 与 NFD2 或 FNFD1 联合使用时，四龄幼虫的 LC50 分别比单独使用 DiPel 低 4.4X 和 3.4X。DiPel 与 NFD2 和 FNFD1 联合使用时，四龄幼虫的 LC50 值比单独使用 DiPel 低 5.0X。当 XenTari 与 NFD2 或 FNFD1 联合使用时，四龄幼虫的 LC50 分别比单独使用 XenTari 低 5.7X 和 3.3X。XenTari 与 NFD2 和 FNFD1 联合使用时，四龄幼虫的 LC50 值比单独使用 XenTari 低 6.7X。
