Medical and Veterinary Entomology Department-Centro Agricoltura Ambiente Giorgio Nicoli, Via Argini Nord 3351, 40014 Crevalcore (BO), Italy.
J Econ Entomol. 2011 Feb;104(1):266-73. doi: 10.1603/ec10108.
To set up a sterile male technique program to control Aedes albopictus (Skuse) in areas in northern Italy, a pilot mass-rearing facility is under development. For this purpose, experiments were carried out to find the optimal larval density for the optimization of the rearing parameters, i.e., to obtain the fastest larval development, the highest larval and pupal survival rate, and large-sized pupae. Several different larval densities, from 40 to 2874 larvae per liter, were tested. For densities from 40 to 600 larvae per liter significant size differences were found among pupae obtained under different larval densities. The larvae raised at the lowest density tended to be smaller and to develop most slowly, i.e., longer pupation time. Also, increasing water volume and depth seemed to negatively affect the pupation success. Compared with the other larval densities tested, the larvae reared at a density of 2874 larvae per liter developed slightly faster and showed higher survival rates, indicating this density as appropriate for the development of a mass rearing, at least using the current larval diet.
为了在意大利北部地区建立一个无菌雄性技术项目来控制白纹伊蚊（Skuse），正在开发一个试点大规模饲养设施。为此，进行了实验以找到最佳的幼虫密度，从而优化饲养参数，即获得最快的幼虫发育速度、最高的幼虫和蛹存活率以及大尺寸的蛹。测试了从 40 到 2874 条幼虫/升的几种不同幼虫密度。在 40 到 600 条幼虫/升的密度下，在不同幼虫密度下获得的蛹之间存在显著的大小差异。在最低密度下饲养的幼虫往往较小，发育最慢，即化蛹时间较长。此外，增加水量和水深似乎会对化蛹成功率产生负面影响。与测试的其他幼虫密度相比，在 2874 条幼虫/升的密度下饲养的幼虫发育速度略快，存活率更高，表明这种密度适合大规模饲养的发展，至少使用当前的幼虫饮食是合适的。
