Balestrino Fabrizio, Puggioli Arianna, Malfacini Marco, Albieri Alessandro, Carrieri Marco, Bouyer Jeremy, Bellini Romeo
Centro Agricoltura Ambiente "G. Nicoli", Sanitary Entomology and Zoology Department, Crevalcore, Italy.
FAO/IAEA Insect Pest Control Laboratory (IPCL), FAO/IAEA Agriculture and Biotechnology Laboratories, FAO/IAEA Joint Division of Nuclear Techniques in Food and Agriculture (NAFA), Vienna, Austria.
Front Bioeng Biotechnol. 2022 Jul 19;10:876677. doi: 10.3389/fbioe.2022.876677. eCollection 2022.
Mark-release-recapture (MRR) trials have been conducted in Northern Italy to evaluate the capacity of radio-substerilized males to survive, disperse, and engage in mating in the field. Two MRR sessions with the human landing collection method (HLC) were conducted with the simultaneous release of irradiated males marked with four different pigment colors. The survival and dispersal rates seem to be influenced more by environmental factors such as barriers, shading, and vegetation rather than weather parameters. In this study, we confirmed a positive linear relationship between the sterile adult male's daily survival rate and the relative humidity previously reported in similar experimental conditions and a different dispersal capacity of the released males in low- (NDVI index <0.4) and high (NDVI index >0.4)-vegetated areas. Consistent with previous studies, males have their maximal dispersion in the first days after release, while in the following days the males become more stationary. The similar field performances obtained with marked and unmarked radio-sterilized and untreated males on similar environments confirm the negligible effects of irradiation and marking procedures on the quality of the males released. The similar sterile to wild (S/W) male ratio measured in high- and low-vegetation areas in the release sites indicates a similar distribution pattern for the wild and the released sterile males. According to the MRR data collected, the Lincoln index estimated different mean population densities in the study areas equal to 7,000 and 3,000 male/ha, respectively.
在意大利北部进行了标记重捕（MRR）试验，以评估经放射性亚不育处理的雄性在野外的生存、扩散及交配能力。采用人工诱捕收集法（HLC）进行了两次MRR试验，同时释放了用四种不同色素颜色标记的经辐照雄性。生存和扩散率似乎更多地受到诸如障碍物、遮荫和植被等环境因素的影响，而非天气参数。在本研究中，我们证实了在类似实验条件下，不育成年雄性的每日生存率与先前报道的相对湿度之间存在正线性关系，以及在低植被区（归一化植被指数（NDVI）<0.4）和高植被区（NDVI>0.4）释放的雄性具有不同的扩散能力。与先前研究一致，雄性在释放后的头几天扩散能力最强，而在随后几天则变得更加静止。在相似环境中，标记和未标记的经放射性不育处理及未处理的雄性表现出相似的野外性能，这证实了辐照和标记程序对释放雄性质量的影响可忽略不计。在释放地点的高植被区和低植被区测得的不育与野生（S/W）雄性比例相似，表明野生和释放的不育雄性具有相似的分布模式。根据收集到的MRR数据，林肯指数估计研究区域内的平均种群密度分别为7000只/公顷和3000只/公顷。
