Velo Enkelejda, Balestrino Fabrizio, Kadriaj Përparim, Carvalho Danilo Oliveira, Dicko Ahmadou, Bellini Romeo, Puggioli Arianna, Petrić Dusan, Michaelakis Antonios, Schaffner Francis, Almenar David, Pajovic Igor, Beqirllari Alfred, Ali Migel, Sino Gjergji, Rogozi Elton, Jani Vjola, Nikolla Adelina, Porja Tanja, Goga Thanas, Fălcuă Elena, Kavran Mihaela, Pudar Dubravka, Mikov Ognyan, Ivanova-Aleksandrova Nadya, Cvetkovikj Aleksandar, Akıner Muhammet Mustafa, Mikovic Rados, Tafaj Lindita, Bino Silva, Bouyer Jeremy, Mamai Wadaka
Department of Epidemiology and Control of Infectious Diseases, Institute of Public Health, Tirana, Albania.
Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, Vienna, Austria.
Front Bioeng Biotechnol. 2022 Aug 16;10:833698. doi: 10.3389/fbioe.2022.833698. eCollection 2022.
The pathogen transmitting mosquito is spreading rapidly in Europe, putting millions of humans and animals at risk. This species is well-established in Albania since its first detection in 1979. The sterile insect technique (SIT) is increasingly gaining momentum worldwide as a component of area-wide-integrated pest management. However, estimating how the sterile males will perform in the field and the size of target populations is crucial for better decision-making, designing and elaborating appropriate SIT pilot trials, and subsequent large-scale release strategies. A mark-release-recapture (MRR) experiment was carried out in Albania within a highly urbanized area in the city of Tirana. The radio-sterilized adults of Albania strain males were transported by plane from Centro Agricoltura Ambiente (CAA) mass-production facility (Bologna, Italy), where they were reared. In Albania, sterile males were sugar-fed, marked with fluorescent powder, and released. The aim of this study was to estimate, under field conditions, their dispersal capacity, probability of daily survival and competitiveness, and the size of the target population. In addition, two adult mosquito collection methods were also evaluated: BG-Sentinel traps baited with BG-Lure and CO (BGS) versus human landing catch (HLC). The overall recapture rates did not differ significantly between the two methods (2.36% and 1.57% of the total male released were recaptured respectively by BGS and HLC), suggesting a similar trapping efficiency under these conditions Sterile males traveled a mean distance of 93.85 ± 42.58 m and dispersed up to 258 m. Moreover, they were observed living in the field up to 15 days after release with an average life expectancy of 4.26 ± 0.80 days. Whether mosquitoes were marked with green, blue, yellow, or pink, released at 3.00 p.m. or 6.00 p.m., there was no significant difference in the recapture, dispersal, and survival rates in the field. The Fried competitiveness index was estimated at 0.28. This mark-release-recapture study provided important data for better decision-making and planning before moving to pilot SIT trials in Albania. Moreover, it also showed that both BG-traps and HLC were successful in monitoring adult mosquitoes and provided similar estimations of the main entomological parameters needed.
传播病原体的蚊子正在欧洲迅速蔓延，使数百万人类和动物面临风险。自1979年首次发现以来，该物种在阿尔巴尼亚已稳固存在。作为区域综合虫害管理的一部分，昆虫不育技术（SIT）在全球范围内越来越受到关注。然而，估计不育雄蚊在野外的表现以及目标种群的规模对于更好地决策、设计和开展适当的SIT试点试验以及后续的大规模释放策略至关重要。在阿尔巴尼亚地拉那市一个高度城市化的地区进行了一次标记释放再捕获（MRR）实验。阿尔巴尼亚品系雄蚊的辐射不育成虫由飞机从其饲养地Centro Agricoltura Ambiente（CAA）大规模生产设施（意大利博洛尼亚）运来。在阿尔巴尼亚，不育雄蚊以糖水为食，用荧光粉标记后释放。本研究的目的是在野外条件下估计它们的扩散能力、每日存活概率和竞争力以及目标种群的规模。此外，还评估了两种成年蚊子收集方法：用BG引诱剂和CO诱饵的BG-Sentinel诱捕器（BGS）与人诱捕法（HLC）。两种方法的总体再捕获率没有显著差异（BGS和HLC分别再捕获了释放雄蚊总数的2.36%和1.57%），表明在这些条件下诱捕效率相似。不育雄蚊平均飞行距离为93.85±42.58米，扩散距离可达258米。此外，观察到它们在释放后在野外存活长达15天，平均寿命为4.26±0.80天。无论蚊子用绿色、蓝色、黄色还是粉色标记，在下午3点还是6点释放，野外的再捕获率、扩散率和存活率均无显著差异。弗里德竞争力指数估计为0.28。这项标记释放再捕获研究为在阿尔巴尼亚开展SIT试点试验之前进行更好的决策和规划提供了重要数据。此外，研究还表明，BG诱捕器和人诱捕法在监测成年蚊子方面都很成功，并能提供所需主要昆虫学参数的相似估计值。
