Department of Materials and Interfaces, the Weizmann Institute of Science, Rehovot, Israel.
Phys Rev Lett. 2011 Jan 28;106(4):048104. doi: 10.1103/PhysRevLett.106.048104. Epub 2011 Jan 24.
A complete gene expression reaction is reconstituted in a cell-free system comprising the entire endogenous transcription, translation, as well as mRNA and protein degradation machinery of E. coli. In dissecting the major reaction steps, we derive a coarse-grained enzymatic description of biosynthesis and degradation, from which ten relevant rate constants and concentrations are determined. Governed by zeroth-order degradation, protein expression follows a sharp transition from undetectable levels to constant-rate accumulation, without reaching steady state.
在一个无细胞系统中，完整的基因表达反应是由大肠杆菌的整个内源性转录、翻译以及 mRNA 和蛋白质降解机制组成的。在剖析主要反应步骤时，我们从生物合成和降解的粗粒度酶描述中得出了十个相关的速率常数和浓度。由于零级降解，蛋白质表达从无法检测到的水平急剧转变为恒速积累，而不会达到稳定状态。
