Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan.
Mitochondrion. 2011 Jul;11(4):575-86. doi: 10.1016/j.mito.2011.03.002. Epub 2011 Mar 23.
Mitochondrial DNA (mtDNA) is generally packaged into the mitochondrial nucleoid (mt-nucleoid) by a high-mobility group (HMG) protein. Glom is an mtDNA-packaging HMG protein in Physarum polycephalum. Here we identified a new mtDNA-packaging protein, Glom2, which had a region homologous with yeast Mgm101. Glom2 could bind to an entire mtDNA and worked synergistically with Glom for condensation of mtDNA in vitro. Down-regulation of Glom2 enhanced the alteration of mt-nucleoid morphology and the loss of mtDNA induced by down-regulation of Glom, and impaired mRNA accumulation of some mtDNA-encoded genes. These data suggest that Glom2 may organize the mt-nucleoid coordinately with Glom.
线粒体 DNA（mtDNA）通常由一个高迁移率族（HMG）蛋白包装到线粒体类核（mt-nucleoid）中。在多头绒泡菌（Physarum polycephalum）中，Glom 是一种 mtDNA 包装 HMG 蛋白。在这里，我们鉴定了一种新的 mtDNA 包装蛋白 Glom2，它与酵母 Mgm101 有同源区域。Glom2 可以结合整个 mtDNA，并与 Glom 协同作用，在体外促进 mtDNA 的浓缩。下调 Glom2 增强了 mt-nucleoid 形态的改变和 Glom 下调诱导的 mtDNA 丢失，并损害了一些 mtDNA 编码基因的 mRNA 积累。这些数据表明，Glom2 可能与 Glom 一起协调组织 mt-nucleoid。
