Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
J Proteomics. 2011 Aug 12;74(8):1351-63. doi: 10.1016/j.jprot.2011.03.002. Epub 2011 Mar 23.
Boron (B) deficiency greatly limits plants' growth and development. Since the root is the organ that first senses the deficiency, we have analyzed the adaptive responses of Lupinus albus roots to long-term B deficiency. Large morphological differences were observed between plants grown with or without B, and 265 polypeptides were found to be responsive to B deficiency out of a total of 406 polypeptides detected by two-dimensional electrophoresis in the L. albus root proteome. By using mass spectrometry techniques we were able to securely identify 128 of the responsive polypeptides that are related to cell wall metabolism, cell structure, defense, energy pathways and protein metabolism. The detection of multiple peptide isoforms is striking, suggesting that protein modification may have an important contribution during the plant response to long-term B deficiency. Furthermore, detected changes in cytoskeletal associated proteins indicate altered cytoskeletal biosynthesis and suggest that B may have an important contribution in this process.
硼（B）缺乏极大地限制了植物的生长和发育。由于根是最先感知到缺乏的器官，因此我们分析了 Lupinus albus 根对长期 B 缺乏的适应反应。有 B 和没有 B 的植物之间观察到了明显的形态差异，在 Lupinus albus 根蛋白质组中通过二维电泳检测到的 406 种多肽中，有 265 种对 B 缺乏有反应。通过使用质谱技术，我们能够可靠地鉴定出 128 种与细胞壁代谢、细胞结构、防御、能量途径和蛋白质代谢有关的响应多肽。对多种肽同工型的检测引人注目，表明蛋白质修饰可能在植物对长期 B 缺乏的反应中具有重要贡献。此外，检测到细胞骨架相关蛋白的变化表明细胞骨架生物合成发生了改变，表明 B 可能在这个过程中具有重要作用。
