College of Resources and Environmental Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Horticultural Plant Physiology, Biochemistry and Molecular Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
J Proteomics. 2013 Nov 20;93:179-206. doi: 10.1016/j.jprot.2013.04.025. Epub 2013 Apr 28.
Seedlings of Citrus sinensis were fertilized with boron (B)-deficient (0μM H3BO3) or -sufficient (10μM H3BO3) nutrient solution for 15weeks. Thereafter, iTRAQ analysis was employed to compare the abundances of proteins from B-deficient and -sufficient roots. In B-deficient roots, 164 up-regulated and 225 down-regulated proteins were identified. These proteins were grouped into the following functional categories: protein metabolism, nucleic acid metabolism, stress responses, carbohydrate and energy metabolism, cell transport, cell wall and cytoskeleton metabolism, biological regulation and signal transduction, and lipid metabolism. The adaptive responses of roots to B-deficiency might include following several aspects: (a) decreasing root respiration; (b) improving the total ability to scavenge reactive oxygen species (ROS); and (c) enhancing cell transport. The differentially expressed proteins identified by iTRAQ are much larger than those detected using 2D gel electrophoresis, and many novel B-deficiency-responsive proteins involved in cell transport, biological regulation and signal transduction, stress responses and other metabolic processes were identified in this work. Our results indicate remarkable metabolic flexibility of citrus roots, which may contribute to the survival of B-deficient plants. This represents the most comprehensive analysis of protein profiles in response to B-deficiency.
In this study, we identified many new proteins involved in cell transport, biological regulation and signal transduction, stress responses and other metabolic processes that were not previously known to be associated with root B-deficiency responses. Therefore, our manuscript represents the most comprehensive analysis of protein profiles in response to B-deficiency and provides new information about the plant response to B-deficiency. This article is part of a Special Issue entitled: Translational Plant Proteomics.
将甜橙幼苗用硼（B）缺乏（0μM H3BO3）或充足（10μM H3BO3）营养液处理 15 周。此后，采用 iTRAQ 分析比较 B 缺乏和充足根系的蛋白质丰度。在 B 缺乏的根中，鉴定出 164 个上调和 225 个下调的蛋白质。这些蛋白质被分为以下功能类别：蛋白质代谢、核酸代谢、应激反应、碳水化合物和能量代谢、细胞转运、细胞壁和细胞骨架代谢、生物调节和信号转导以及脂质代谢。根对 B 缺乏的适应反应可能包括以下几个方面：（a）降低根呼吸；（b）提高总清除活性氧（ROS）的能力；和（c）增强细胞转运。iTRAQ 鉴定的差异表达蛋白比 2D 凝胶电泳检测的多得多，并且在这项工作中鉴定了许多涉及细胞转运、生物调节和信号转导、应激反应和其他代谢过程的新的 B 缺乏响应蛋白。我们的结果表明，柑橘根具有显著的代谢灵活性，这可能有助于 B 缺乏植物的存活。这代表了对 B 缺乏响应的蛋白质图谱的最全面分析。
在这项研究中，我们鉴定了许多新的蛋白质，这些蛋白质涉及细胞转运、生物调节和信号转导、应激反应和其他代谢过程，以前未知与根 B 缺乏反应有关。因此，我们的论文代表了对 B 缺乏响应的蛋白质图谱的最全面分析，并提供了有关植物对 B 缺乏响应的新信息。本文是题为“转化植物蛋白质组学”的特刊的一部分。
