Komlos L, Vardimon D, Notmann J, Zamir R, Klein T, Livni E, Hart J, Goldman J, Halbrecht I
Gattegno Research Institute of Human Reproduction and Fetal Development, Hasharon Hospital, Petah-Tiqva, Israel.
Am J Reprod Immunol. 1990 Jan-Feb;22(1-2):4-8. doi: 10.1111/j.1600-0897.1990.tb01024.x.
One-way mixed mother-child lymphocyte cultures (MMCLC) 4 to 20 years after the last delivery were studied with maternal responding and children's or fathers' stimulating cells (MMFLC) in 14 multiple child families with 18 sons and 20 daughters. HLA antigen typing locus A, B, DR was performed for all family members. As reported previously for newborn cells, a significantly increased maternal response could be observed in MMCLC with male as compared to female children's stimulating cells. Although the number of cases studied was small, it seems that the increased stimulating effect of male children's cells could also be observed when MMCLC values from children of different sex, and identical A,B,DR haplotypes were compared. In contrast to this, A,B,DR haploidentical children of the same sex seem to have a similar stimulating effect on the maternal response in MMCLC. The results suggest that male children's Y-chromosome-correlated minor histocompatibility antigens may additionally stimulate the maternal immune response in MMCLC.
对14个多子女家庭（其中有18个儿子和20个女儿）在最后一次分娩后4至20年进行单向混合母婴淋巴细胞培养（MMCLC）研究，以母亲的细胞作为反应细胞，以子女或父亲的细胞作为刺激细胞（MMFLC）。对所有家庭成员进行了HLA抗原A、B、DR位点分型。如先前对新生儿细胞的报道，与以女性子女的细胞作为刺激细胞相比，在以男性子女的细胞作为刺激细胞的MMCLC中可观察到母亲的反应显著增强。尽管研究的病例数量较少，但当比较不同性别、具有相同A、B、DR单倍型的子女的MMCLC值时，似乎也能观察到男性子女细胞的刺激作用增强。与此相反，相同性别的A、B、DR单倍型相同的子女似乎对MMCLC中母亲的反应具有相似的刺激作用。结果表明，男性子女与Y染色体相关的次要组织相容性抗原可能会额外刺激MMCLC中母亲的免疫反应。
