Pappolla M A, Alzofon J, McMahon J, Theodoropoulos T J
Department of Pathology, Veterans Administration Medical Center, Montrose, New York.
Eur Arch Psychiatry Neurol Sci. 1990;239(5):314-9. doi: 10.1007/BF01735057.
The ultrastructure of Alzheimer's neurofibrillary tangles is heterogeneous and includes abnormal paired helical filaments (PHF) and various other insoluble structures. Insoluble non-PHF components isolated from neurofibrillary tangles were examined by electron microscopy. Comparison of these fractions with normal assembled neurofilaments and normal brain microtubules revealed scattered profiles which were morphologically (not chemically) identical to structures present in the microtubule, but not in the neurofilament preparations. These results support the notion that insoluble microtubules contribute to the make up of the neurofibrillary tangle. Based on these findings, preliminary experiments were conducted which suggest that non-enzymatic glycosylation may be a pathway leading to insolubility of the microtubules.
阿尔茨海默病神经原纤维缠结的超微结构具有异质性，包括异常的双螺旋丝（PHF）和各种其他不溶性结构。通过电子显微镜检查了从神经原纤维缠结中分离出的不溶性非PHF成分。将这些组分与正常组装的神经丝和正常脑微管进行比较，发现了一些分散的轮廓，这些轮廓在形态上（而非化学上）与微管中存在的结构相同，但在神经丝制剂中不存在。这些结果支持了不溶性微管参与神经原纤维缠结组成的观点。基于这些发现，进行了初步实验，结果表明非酶糖基化可能是导致微管不溶性的一条途径。
