Perry G, Rizzuto N, Autilio-Gambetti L, Gambetti P
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3916-20. doi: 10.1073/pnas.82.11.3916.
Neurofibrillary tangles from Alzheimer disease patients share antigenic determinants with neurofilaments and microtubule-associated proteins, as shown by light microscopy immunocytology. The present study addresses the issue of whether these determinants are located on the paired helical filaments or on other components of the neurofibrillary tangle. Sections from postmortem brains from Alzheimer disease patients were stained by using Bodian's silver method or immunostained by using poly- and monoclonal antibodies to neurofilaments and polyclonal antibodies to microtubules. Bodian's silver stain has an intense affinity for neurofibrillary tangles and has been shown to bind to specific domains of neurofilament subunits. The antibodies to neurofilaments used here immunostain most or all of the neurofibrillary tangles present in the sections whereas the antiserum to microtubule protein immunoreacted with about half of the neurofibrillary tangles. All of the antibodies as well as Bodian's silver stain reacted with the paired helical filaments. The epitopes that we have shown to be present in the paired helical filament, in contrast to the corresponding epitopes present in normal neuronal cytoskeleton, are insoluble in ionic detergent. It is concluded that these epitopes are integral components of the paired helical filaments and that, at least in part, paired helical filaments are derived from altered elements of the normal neuronal cytoskeleton.
如光学显微镜免疫细胞学所示，阿尔茨海默病患者的神经原纤维缠结与神经丝和微管相关蛋白具有共同的抗原决定簇。本研究探讨了这些决定簇是位于双螺旋丝上还是位于神经原纤维缠结的其他成分上。使用博迪安银染法对阿尔茨海默病患者死后大脑的切片进行染色，或使用针对神经丝的多克隆和单克隆抗体以及针对微管的多克隆抗体进行免疫染色。博迪安银染法对神经原纤维缠结具有强烈的亲和力，并且已被证明可与神经丝亚基的特定结构域结合。此处使用的针对神经丝的抗体对切片中存在的大多数或所有神经原纤维缠结进行免疫染色，而针对微管蛋白的抗血清与大约一半的神经原纤维缠结发生免疫反应。所有抗体以及博迪安银染法均与双螺旋丝发生反应。与正常神经元细胞骨架中存在的相应表位相比，我们已证明存在于双螺旋丝中的表位不溶于离子去污剂。得出的结论是，这些表位是双螺旋丝的组成成分，并且双螺旋丝至少部分源自正常神经元细胞骨架的改变成分。
