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注射到非洲爪蟾受精卵中的非洲爪蟾珠蛋白基因的复制与表达。

Replication and expression of Xenopus laevis globin genes injected into fertilized Xenopus eggs.

作者信息

Bendig M M, Williams J G

出版信息

Proc Natl Acad Sci U S A. 1983 Oct;80(20):6197-201. doi: 10.1073/pnas.80.20.6197.

DOI:10.1073/pnas.80.20.6197
PMID:6578503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC394262/
Abstract

Cloned Xenopus adult alpha 1- and beta 1-globin genes were injected into fertilized Xenopus eggs, and the eggs were allowed to develop into swimming tadpoles. The injected DNA replicated during early Xenopus development but did not become methylated de novo. When DNA was modified with Hpa II methylase before injection, methylation was maintained during replication. Although the Xenopus adult globin genes are not normally expressed until metamorphosis, both the unmethylated and in vitro methylated adult alpha- and beta-globin genes were transcribed at low levels, but from the correct promoters, during early development.

摘要

将克隆的非洲爪蟾成年α1和β1珠蛋白基因注射到非洲爪蟾受精卵中，然后让这些卵发育成能游动的蝌蚪。注入的DNA在非洲爪蟾早期发育过程中进行复制，但未发生从头甲基化。在注射前用Hpa II甲基化酶修饰DNA时，甲基化在复制过程中得以维持。尽管非洲爪蟾成年珠蛋白基因通常要到变态期才表达，但未甲基化的和体外甲基化的成年α和β珠蛋白基因在早期发育过程中均以低水平转录，且转录起始于正确的启动子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/b6816381f162/pnas00646-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/e492ac93a561/pnas00646-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/0d1bea36e86d/pnas00646-0059-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/07905e032fed/pnas00646-0059-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/c0e69a082675/pnas00646-0059-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/ee8b295762fa/pnas00646-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/b6816381f162/pnas00646-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/e492ac93a561/pnas00646-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/0d1bea36e86d/pnas00646-0059-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/07905e032fed/pnas00646-0059-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/c0e69a082675/pnas00646-0059-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/ee8b295762fa/pnas00646-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab36/394262/b6816381f162/pnas00646-0060-b.jpg

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本文引用的文献

1
In vitro methylation of the BsuRI (5'-GGCC-3') sites in the E2a region of adenovirus type 2 DNA does not affect expression in Xenopus laevis oocytes.对2型腺病毒DNA的E2a区域中的BsuRI（5'-GGCC-3'）位点进行体外甲基化处理，并不会影响其在非洲爪蟾卵母细胞中的表达。
Mol Cell Biol. 1982 Dec;2(12):1574-80. doi: 10.1128/mcb.2.12.1574-1580.1982.
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Linkage of adult alpha- and beta-globin genes in X. laevis and gene duplication by tetraploidization.非洲爪蟾中成年α-珠蛋白基因和β-珠蛋白基因的连锁以及四倍体化导致的基因复制。
Cell. 1980 Sep;21(2):555-64. doi: 10.1016/0092-8674(80)90493-6.
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Trans-complementable copy-number mutants of plasmid ColE1.
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An embryonic demethylation mechanism involving binding of transcription factors to replicating DNA.一种涉及转录因子与复制DNA结合的胚胎去甲基化机制。
EMBO J. 1998 Mar 2;17(5):1446-53. doi: 10.1093/emboj/17.5.1446.
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Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.将非洲爪蟾蝌蚪和成体的β-珠蛋白基因注入非洲爪蟾受精卵后二者的差异表达。
Mol Cell Biol. 1984 Mar;4(3):567-70. doi: 10.1128/mcb.4.3.567-570.1984.
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Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs.注射到非洲爪蟾卵母细胞和未受精卵中的非洲爪蟾珠蛋白基因的转录保真度。
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Nucleic Acids Res. 1984 Mar 12;12(5):2283-302. doi: 10.1093/nar/12.5.2283.
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Growth conditions of F9 embryonal carcinoma cells affect the degree of DNA methylation.F9胚胎癌细胞的生长条件会影响DNA甲基化程度。
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4
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9
Expression of a cloned adenovirus gene is inhibited by in vitro methylation.克隆腺病毒基因的表达受到体外甲基化的抑制。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1073-7. doi: 10.1073/pnas.79.4.1073.
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Transformation of frog embryos with a rabbit beta-globin gene.用兔β-珠蛋白基因对蛙胚胎进行转化。
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