Department of Life Science and Engineering, Hexi University, Zhangye, 734000, China.
Plant Cell Rep. 2011 Aug;30(8):1475-83. doi: 10.1007/s00299-011-1056-4. Epub 2011 Mar 16.
We examined ameliorative effects of salicylic acid (SA) on two cadmium (Cd)-stressed legume crops with different Cd tolerances, viz. Phaseolus aureus (Cd sensitive) and Vicia sativa (Cd tolerant). Cd at 50 μM significantly increased the production of hydrogen peroxide (H(2)O(2)) and superoxide anion (O(2)(·-) ) in root apoplasts of P. aureus and V. sativa. When comparing the two species, we determined that Cd-induced production of H(2)O(2) and O(2)(·-) was more pronounced in P. aureus root apoplasts than in V. sativa root apoplasts. V. sativa had higher activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) than P. aureus in root symplasts and apoplasts. Seed-soaking pretreatment with 100 μM SA decreased Cd-induced production of H(2)O(2) and O(2)(·-) in apoplasts of both species, and increased activities of symplastic and apoplastic SOD, symplastic APX, and apoplastic CAT under Cd stress. Hence, SA-induced Cd tolerances in P. aureus and V. sativa are likely associated with increases in symplastic and apoplastic antioxidant enzyme activities.
我们研究了水杨酸（SA）对两种具有不同镉耐性的豆科作物（Cd 敏感的 Phaseolus aureus 和 Cd 耐受的 Vicia sativa）的改良作用。50μM 的 Cd 显著增加了 P. aureus 和 V. sativa 根质外体中过氧化氢（H2O2）和超氧阴离子（O2（·-））的产生。在比较这两个物种时，我们确定 Cd 诱导的 H2O2 和 O2（·-）的产生在 P. aureus 根质外体中比在 V. sativa 根质外体中更为明显。V. sativa 根质外体和质体内的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）的活性均高于 P. aureus。用 100μM SA 对种子进行浸种预处理可降低两种物种质外体中 Cd 诱导的 H2O2 和 O2（·-）的产生，并在 Cd 胁迫下增加质体和质外体 SOD、质体 APX 和质外体 CAT 的活性。因此，SA 诱导的 P. aureus 和 V. sativa 的 Cd 耐性可能与质体和质外体抗氧化酶活性的增加有关。
