López Rodrigo, Dahlén Gunnar, Retamales Carolina, Baelum Vibeke
Department of Periodontology, Faculty of Health Sciences, Aarhus University, Denmark.
Eur J Oral Sci. 2011 Apr;119(2):141-50. doi: 10.1111/j.1600-0722.2011.00808.x. Epub 2011 Mar 3.
It has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. It is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. To investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 18 microbial species using the DNA-DNA hybridization technique. Actinomyces oris accounted for the highest proportion of the flora and was more predominant among controls. Prevotella nigrescens, Prevotella intermedia, Porphyromonas gingivalis, and Tannerella forsythia were present at higher levels among the subjects with periodontitis. Factor analyses identified one factor characterized by highly positive loadings for T. forsythia, Campylobacter rectus, P. gingivalis, P. intermedia, P. nigrescens, Parvimonas micra, and Treponema denticola, and another factor characterized by highly positive loadings of A. oris, Capnocytophaga ochracea, Eikenella corrodens, Streptococcus intermedius, Selenomonas noxia, Streptococcus oralis, Streptococcus sanguinis, and Veillonella parvula. Aggregatibacter actinomycetemcomitans and Streptococcus mutans did not load on any of the two factors, while Fusobacterium nucleatum loaded on both. These findings confirm the occurrence of clustering of subgingival bacteria according to case status also among young individuals.
越来越多的人认识到，成年牙周炎患者的龈下菌斑中微生物群体会相互作用。然而，与牙周炎相关的微生物群落在牙周炎早期和更严重病例中是否存在差异，目前尚不清楚。为了进一步研究这一点，从87名患有牙周炎的青少年和73名对照者的六个部位采集龈下菌斑，并使用DNA-DNA杂交技术对样本进行分析，以检测18种微生物。口腔放线菌在菌群中所占比例最高，在对照组中更为常见。变黑普雷沃菌、中间普雷沃菌、牙龈卟啉单胞菌和福赛坦纳菌在牙周炎患者中的含量较高。因子分析确定了一个因子，其特征是福赛坦纳菌、直肠弯曲菌、牙龈卟啉单胞菌、中间普雷沃菌、变黑普雷沃菌、微小单胞菌和具核梭杆菌的负荷高度为正，另一个因子的特征是口腔放线菌、赭色二氧化碳嗜纤维菌、腐蚀艾肯菌、中间链球菌、有害月形单胞菌、口腔链球菌、血链球菌和小韦荣球菌的负荷高度为正。伴放线聚集杆菌和变形链球菌未在这两个因子中的任何一个上有负荷，而具核梭杆菌在两个因子上均有负荷。这些发现证实了龈下细菌根据病例状态的聚类在年轻人中也存在。
