Rey Diego, Areces Cristina, Parga-Lozano Carlos, Gómez-Prieto Pablo, Crawford Michael H, Arnaiz-Villena Antonio
Department of Immunology, Madrid Regional Blood Center, Facultad de Medicina, Universidad Complutense de Madrid, Avenida Complutense s/n, 28040 Madrid, Spain.
Hum Biol. 2010 Dec;82(5-6):737-44. doi: 10.3378/027.082.0513.
We typed a subset of the Aleut population for HLA loci (HLA-A, HLA-B, HLA-DRB1, HLA-DQB1) to obtain an HLA profile, which was compared to other Eurasian and Amerindian populations for studying Aleut origin and its significance on the peopling of the Americas. Allele frequencies at the four loci were identified in an Aleut sample using standard indirect DNA sequencing methods. Genetic distances with Amerindians and Eurasians were obtained by comparing Aleut allele frequencies with a worldwide population database (13,164 chromosomes). The most frequently extended HLA haplotypes were also calculated. We also generated Aleut relatedness dendrograms and calculated correspondence relatedness in a multidimensional scale. Both neighbor-joining dendrograms and correspondence analysis separated Aleuts from Eskimos and Amerindians. Aleuts are closer genetically to Europeans, including Scandinavians and English. Our results are concordant with those obtained by Y-chromosome analysis, suggesting that most male Aleut ancestors of our sample came mainly from Europe.
我们对阿留申人群的一个子集进行了HLA基因座（HLA - A、HLA - B、HLA - DRB1、HLA - DQB1）分型，以获得HLA图谱，并将其与其他欧亚和美洲印第安人群进行比较，以研究阿留申人的起源及其对美洲人口迁移的意义。使用标准间接DNA测序方法在一个阿留申样本中确定了四个基因座的等位基因频率。通过将阿留申等位基因频率与一个全球人群数据库（13164条染色体）进行比较，获得了与美洲印第安人和欧亚人的遗传距离。还计算了最常见的扩展HLA单倍型。我们还生成了阿留申人亲缘关系树状图，并在多维尺度上计算了对应亲缘关系。邻接法树状图和对应分析都将阿留申人与爱斯基摩人和美洲印第安人区分开来。阿留申人与包括斯堪的纳维亚人和英国人在内的欧洲人在基因上更为接近。我们的结果与通过Y染色体分析获得的结果一致，表明我们样本中大多数阿留申男性祖先主要来自欧洲。
