Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Biochemistry (Mosc). 2010 Dec;75(13):1517-27. doi: 10.1134/s000629791013002x.
This review analyzes the concept according to which the pathway of synthesized peptide from the ribosome peptidyl transferase center to the exit domain goes along the tunnel of the large subparticle. Experimental data on the accessibility of the nascent polypeptide chain to molecules of modifying agents and fluorescence quenchers are considered. Results of localization of the exit site for the nascent peptide on the ribosome surface, possible conformational states of the peptide, and its mobility and folding on the ribosome are analyzed. The analysis is based on the ribosomal tunnel parameters obtained using X-ray crystallography of whole ribosomes and large ribosomal subparticles. Special attention is given to data that do not fit in the concept of the "tunnel for peptide exit" and to results already obtained before the reliable tunnel visualization using X-ray crystallography was achieved.
本文根据核糖体肽基转移酶中心合成肽的途径沿着大亚基隧道行进的概念进行了分析。考虑了新生多肽链与修饰剂和荧光猝灭剂分子可及性的实验数据。分析了核糖体表面新生肽出口部位的定位、肽的可能构象状态及其在核糖体上的流动性和折叠。该分析基于使用全核糖体和大亚基进行 X 射线晶体学获得的核糖体隧道参数。特别关注不符合“肽出口隧道”概念的数据，以及在使用 X 射线晶体学可靠地可视化隧道之前已经获得的结果。
