Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 ONF, UK.
J Virol Methods. 2011 Jun;174(1-2):42-6. doi: 10.1016/j.jviromet.2011.03.016. Epub 2011 Mar 17.
This paper describes the improvement of a rapid diagnostic test for the detection of rinderpest virus (RPV) at pen-side and the development of a similar test for the detection of another Morbillivirus, peste de petits ruminants virus (PPRV). Using the Svanova Biotech format, prototype chromatographic strip test devices were developed for RPV and PPRV detection. For the RP device, the incorporation of a monoclonal antibody (Mab), which recognises additional RPV strains of RPV lineage 2, enhanced the range of reactivity of the rapid diagnostic test. The device detected antigen in animals infected experimentally with different RPV strains. It also showed detection levels similar to the RP Clearview™ device reported previously. In addition, RPV was also detected under field conditions in Pakistan. A PPRV specific Mab (C77) was used for the development of the PPR test. This Mab recognised a wide range of PPRV isolates and did not show any cross-reactivity with any other virus tested. In animal experiments the device was able to detect viral antigen in eye swabs taken from the animals. The PPRV test should be invaluable for future PPR control eradication programs.
本文描述了一种用于在现场检测牛瘟病毒（RPV）的快速诊断检测方法的改进，以及一种用于检测另一种麻疹病毒——绵羊和山羊瘟病毒（PPRV）的类似检测方法的开发。使用 Svanova Biotech 格式，为 RPV 和 PPRV 检测开发了原型色谱条测试设备。对于 RP 设备，加入了一种单克隆抗体（Mab），它可以识别 RPV 2 谱系的其他 RPV 株，增强了快速诊断检测的反应范围。该设备检测了用不同 RPV 株感染的动物的抗原。它还显示出与先前报道的 RP Clearview™设备相似的检测水平。此外，该设备还在巴基斯坦的现场条件下检测到了 RPV。一种用于开发 PPR 检测的 PPRV 特异性 Mab（C77）。该 Mab 识别广泛的 PPRV 分离株，与测试的任何其他病毒均无交叉反应。在动物实验中，该设备能够从动物的眼拭子中检测到病毒抗原。该 PPRV 检测方法对于未来的 PPR 控制根除计划将非常有价值。
