Schessner M, Schnorr B
Department of Veterinary Anatomy, Histology and Embryology, University of Giessen, FRG.
Acta Anat (Basel). 1990;138(1):50-5. doi: 10.1159/000146919.
The intracellular localization of calcium adenosine triphosphatase (Ca2(+)-ATPase) was studied ultracytochemically in the pyloric glands of the abomasal mucosa of cattle. A remarkable staining pattern exhibited the Golgi apparatus, as there was a gradation in staining of the interior sides of dictyosomal cisternae from the not or weakly stained cis to the heavily stained trans face. Membranes of Golgi-endoplasmic reticulum lysosome complex-secretory vesicles showed either no or strong enzyme activity. Membranes of secretory vesicles accumulated in the cell apex stained positive for ATPase activity. This accounts also for the apical cortical cytoplasm. From these results it is speculated that Ca2(+)-ATPase may play an important role in the pathway of exocytotic secretion, especially in the process of membrane sorting and biogenesis of secretory vesicles, in the steps of vesicle accumulation and transport to the site of exocytosis as well as in membrane fusion events.
采用超微细胞化学方法研究了牛皱胃黏膜幽门腺中钙三磷酸腺苷酶（Ca2(+)-ATP酶）的细胞内定位。一种显著的染色模式显示了高尔基体，因为在高尔基体囊泡潴泡内侧的染色存在从未染色或弱染色的顺面到强染色的反面的梯度变化。高尔基体-内质网-溶酶体复合物-分泌小泡的膜要么没有酶活性，要么具有很强的酶活性。积聚在细胞顶端的分泌小泡膜对ATP酶活性呈阳性染色。这也解释了顶端皮质细胞质的情况。从这些结果推测，Ca2(+)-ATP酶可能在胞吐分泌途径中发挥重要作用，特别是在分泌小泡的膜分选和生物发生过程中，在小泡积聚和运输到胞吐部位的步骤以及膜融合事件中。
