IEMN, Avenue Poincaré, Villeneuve d'Ascq, France.
Lab Chip. 2011 May 7;11(9):1620-8. doi: 10.1039/c0lc00716a. Epub 2011 Mar 21.
We present for the first time an electrowetting on dielectric (EWOD) microfluidic system coupled to a surface-assisted laser desorption-ionization (SALDI) silicon nanowire-based interface for mass spectrometry (MS) analysis of small biomolecules. Here, the transfer of analytes has been achieved on specific locations on the SALDI interface followed by their subsequent mass spectrometry analysis without the use of an organic matrix. To achieve this purpose, a device comprising a digital microfluidic system and a patterned superhydrophobic/superhydrophilic silicon nanowire interface was developed. The digital microfluidic system serves for the displacement of the droplets containing analytes, via an electrowetting actuation, inside the superhydrophilic patterns. The nanostructured silicon interface acts as an inorganic target for matrix-free laser desorption-ionization mass spectrometry analysis of the dried analytes. The proposed device can be easily used to realize several basic operations of a Lab-on-Chip such as analyte displacement and rinsing prior to MS analysis. We have demonstrated that the analysis of low molecular weight compounds (700 m/z) can be achieved with a very high sensitivity (down to 10 fmol μL(-1)).
我们首次提出了一种电润湿（EWOD）微流控系统，该系统与基于硅纳米线的表面辅助激光解吸电离（SALDI）接口耦合，用于对小分子生物分子进行质谱（MS）分析。在这里，通过 SALDI 接口上的特定位置转移分析物，然后对其进行后续的质谱分析，而无需使用有机基质。为了实现这一目的，开发了一种包含数字微流控系统和图案化超亲水/超疏水硅纳米线接口的装置。数字微流控系统通过电润湿致动作用，用于将含有分析物的液滴在超亲水图案内进行位移。纳米结构硅界面作为无基质激光解吸电离质谱分析干燥分析物的无机靶。所提出的装置可用于轻松实现芯片实验室的几个基本操作，例如在 MS 分析之前进行分析物置换和冲洗。我们已经证明，低分子量化合物（700 m/z）的分析可以达到非常高的灵敏度（低至 10 fmol μL(-1)）。
