Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland.
Phys Chem Chem Phys. 2011 Jun 7;13(21):9978-81. doi: 10.1039/c0cp02832k. Epub 2011 Mar 23.
Lipid domains in supported lipid layers serve as a popular model to gain insight into the processes associated with the compartmentalization of biological membranes into so-called lipid rafts. In this paper, we present reproducible tip-enhanced Raman spectra originating from a very small number of molecules in a lipid monolayer on a gold surface, probed by the apex of a nanometer-sized silver tip. For the first time, we show large (128 × 128 pixels), high-resolution (< 50 nm) tip-enhanced Raman images of binary lipid mixtures with full spectral information at each pixel.
脂质双层中的脂质域可用作一种流行的模型，以便深入了解将生物膜分隔成所谓的脂质筏的相关过程。在本文中，我们展示了源自金表面上脂质单层中非常少量分子的可重现的尖端增强拉曼光谱，这些分子是由纳米级银尖端的尖端探测到的。我们首次展示了具有全光谱信息的二元脂质混合物的大（128×128 像素）、高分辨率（<50nm）的尖端增强拉曼图像，每个像素都有完整的光谱信息。
