IPHT-Institute of Photonic Technology, Albert-Einstein-Str. 9, D-07745 Jena, Germany.
Curr Opin Chem Biol. 2011 Oct;15(5):719-24. doi: 10.1016/j.cbpa.2011.06.020. Epub 2011 Jul 19.
Tip-enhanced Raman scattering (TERS) enables the label-free investigation of biochemical interfaces with nanometer lateral resolution by combining the benefits of the intrinsic molecular specificity of Raman spectroscopy, the sensitivity because of signal enhancing capabilities of plasmonic nanoparticles, and the precision of scanning probe microscopy. The structural differentiation of constituents based on inherent molecular information is possible even down to a few nanometer spatial resolution and consequently, nucleobases, proteins, lipids, and carbohydrates can be identified and localized in a single measurement. This has been shown in the last few years for different biological samples ranging from single DNA strand investigations to cell membrane studies.
尖端增强拉曼散射（TERS）通过结合拉曼光谱的固有分子特异性、等离子体纳米粒子的信号增强能力带来的灵敏度，以及扫描探针显微镜的精度这三者的优势，实现了纳米级横向分辨率下的生化界面的无标记研究。即使在几纳米的空间分辨率下，也可以基于固有分子信息对组成部分进行结构区分，因此可以在单次测量中识别和定位核苷酸碱基、蛋白质、脂质和碳水化合物。这在过去几年中已经在不同的生物样本中得到了证实，范围从单个 DNA 链研究到细胞膜研究。
