Cherry Sara
Department of Microbiology, Penn Genome Frontiers Institute, University of Pennsylvania, Philadelphia, PA, USA.
Methods Mol Biol. 2011;721:375-82. doi: 10.1007/978-1-61779-037-9_23.
Since viral pathogens represent a significant threat to human health, a better understanding of the cellular factors that impact infection would facilitate the development of therapeutics. The recent advent of RNA interference (RNAi) technology coupled with the ease and efficiency of RNAi in Drosophila cell culture has led to the widespread use of this experimental system for high-throughput RNAi screening of host factors required for viral infection [Cherry et al., Genes Dev 19:445-452, 2005; Hao et al., Nature 45:890-893, 2008; Sessions et al., Nature 458:1047-1050, 2009]. Here, we describe the use of this system for the identification of host factors that impact viral infection.
由于病毒病原体对人类健康构成重大威胁，更好地了解影响感染的细胞因子将有助于开发治疗方法。RNA干扰（RNAi）技术的最新出现，以及RNAi在果蝇细胞培养中的简便性和高效性，使得该实验系统被广泛用于对病毒感染所需宿主因子进行高通量RNAi筛选[Cherry等人，《基因与发育》19:445 - 452，2005；Hao等人，《自然》45:890 - 893，2008；Sessions等人，《自然》458:1047 - 1050，2009]。在此，我们描述了使用该系统来鉴定影响病毒感染的宿主因子。
