非洲爪蟾卵母细胞中mRNA定位的可视化。
Visualization of mRNA localization in Xenopus oocytes.
作者信息
Gagnon James A, Mowry Kimberly L
机构信息
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA.
出版信息
Methods Mol Biol. 2011;714:71-82. doi: 10.1007/978-1-61779-005-8_5.
Abstract
Visualization of in vivo mRNA localization provides a tool for understanding steps in the mechanism of transport. Here we detail a method of fluorescently labeling mRNA transcripts and microinjecting them into Xenopus laevis oocytes followed with imaging by confocal microscopy. This technique overcomes a significant hurdle of imaging RNA in the frog oocyte while providing a rapid method of visualizing mRNA localization in high resolution.
摘要
体内mRNA定位的可视化提供了一种理解运输机制步骤的工具。在这里，我们详细介绍一种荧光标记mRNA转录本并将其显微注射到非洲爪蟾卵母细胞中，随后通过共聚焦显微镜成像的方法。该技术克服了在蛙卵母细胞中对RNA进行成像的一个重大障碍，同时提供了一种高分辨率可视化mRNA定位的快速方法。
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